Combined effect of pulse electric field and probe ultrasound technologies for obtaining phenolic compounds from orange by-product Razola Díaz, María del Carmen Sevenich, Robert Rossi Ribeiro, Luma Guerra Hernández, Eduardo Jesús Schlüter, Oliver Verardo, Vito Phenolic compounds Citrus by-products Non-thermal technologies Orange peel by-product is a known source of bioactive compounds with several health benefits. This study focuses on optimizing the conditions for pulsed electric field (PEF) technology as a pretreatment to obtain extracts rich in phenolic compounds from the orange peel. A Box-Behnken design of 15 experiments with 3 independent factors (field energy, number of pulses and pulse width) was performed. The phenolic content was extracted by ultrasound technology and analyzed by HPLC-ESI-TOF-MS. Optimum conditions were 1.4 kV/cm and 30 pulses of 110 μs achieving a recovery of 40.8 mg/g dry weight of phenolic compounds, 27.5% higher than obtained without PEF. Besides, hesperidin and narirutin content increased by 29.4 and 38.9%, respectively. For the antioxidant assays also significative increments (≥ 53%) were achieved. The combination of optimized PEF pretreatment with ultrasound extraction has let to obtain orange peel enriched extracts in polyphenols with higher antioxidant activity. 2024-07-16T08:18:58Z 2024-07-16T08:18:58Z 2024-03-11 journal article M.C. Razola-Díaz et al. LWT 198 (2024) 115950. [https://doi.org/10.1016/j.lwt.2024.115950] https://hdl.handle.net/10481/93145 10.1016/j.lwt.2024.115950 eng info:eu-repo/grantAgreement/EC/H2020/817936 http://creativecommons.org/licenses/by-nc/4.0/ open access Atribución-NoComercial 4.0 Internacional Elsevier