Multilocus Sequence Typing analysis of human Campylobacter coli in Granada (Spain) Carrillo-Ávila, J.A. Solorzano Puerto, Antonio Pérez-Ruiz, M. Gutiérrez Fernández, José Objective: Different subtypes of Campylobacter spp. have been associated with diarrhoea and a Multilocus Sequence Typing (MLST) method has been performed for subtyping. In the present work, MLST was used to analyse the genetic diversity of eight strains of Campylobacter coli. Methods: Nineteen genetic markers were amplified for MLST analysis: AnsB, DmsA, ggt, Cj1585c, CJJ81176-1367/1371, Tlp7, cj1321-cj1326, fucP, cj0178, cj0755/cfrA, ceuE, pldA, cstII, cstIII. After comparing the obtained sequences with the Campylobacter MLST database, the allele numbers, sequence types (STs) and clonal complexes (CCs) were assigned. Results: The 8 C. coli isolates yielded 4 different STs belonging to 2 CCs. Seven isolates belong to ST-828 clonal complex and only one isolate belong to ST-21. Two samples came from the same patient, but were isolated in two different periods of time. Conclusions: MLST can be useful for taxonomic characterization of C. coli isolates. 2024-07-12T09:58:04Z 2024-07-12T09:58:04Z 2016-12 journal article Carrillo-Ávila JA, Sorlózano-Puerto A, Pérez-Ruiz M, Gutiérrez-Fernández J. Multilocus Sequence Typing analysis of human Campylobacter coli in Granada (Spain). Rev Esp Quimioter. 2016 Dec;29(6):332-335. https://hdl.handle.net/10481/93096 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Rev Esp Quimioter .