Evolution of Antibiotic Resistance in Escherichia coli and Klebsiella pneumoniae from Urine Cultures López-Sampedro, Inmaculada Hernández-Chico, Itahisa Gómez-Vicente, Esther Expósito-Ruiz, Manuela Navarro-Marí, José María Gutiérrez Fernández, José Objective: Determine the evolution of antibiotic resistance of symptomatic bacteriuria caused by Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) in Granada. Material and method: A descriptive retrospective study was carried out, including antibiograms of urine cultures in which microorganisms identified as E. coli and K. pneumoniae, were isolated in the Microbiology laboratory of the Hospital Universitario Virgen de las Nieves (Granada, Spain) between January 2016 and June 2021. Results: E. coli was the most frequent isolate (10,048) and its resistance to ampicillin (59.45%) and ticarcillin (59.59%), and the increase to cefepime (15.07%) and amoxicillin-clavulanic acid (17.67%) is noteworthy. K. pneumoniae (2222) is notable for resistance to Fosfomycin (27.91%) and an increase to ciprofloxacin (37.79%) and amoxicillin-clavulanic acid (36.63%). Resistance is generally higher in hospitalized patients, males, and adults. Conclusions: Antibiotic resistance to the studied Enterobacteriaceae is on the rise, requiring empirical treatment targeted to the population area. 2024-06-04T08:16:32Z 2024-06-04T08:16:32Z 2023-05 journal article López-Sampedro I, Hernández-Chico I, Gómez-Vicente E, Expósito-Ruiz M, Navarro-Marí JM, Gutiérrez-Fernández J. Evolution of Antibiotic Resistance in Escherichia coli and Klebsiella pneumoniae from Urine Cultures. Arch Esp Urol. 2023 May;76(3):203-214. doi: 10.56434/j.arch.esp.urol.20237603.24. https://hdl.handle.net/10481/92296 10.56434/j.arch.esp.urol.20237603.24 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Arch Esp Urol .