A Novel Plant-Based Nutraceutical Combined with Exercise Can Revert Oxidative Status in Plasma and Liver in a Diet-Induced-Obesity Animal Model Guzmán Carrasco, Ana Isabel Kapravelou, Garyfallia López-Jurado Romero De La Cruz, María Bermúdez, Francisco Andrés León, Eduardo Terrón Camero, Laura Carmen Prados Salazar, José Carlos Melguizo Alonso, Consolación Porres Foulquie, Jesús María Martínez Martínez, Rosario Obesity Oxidative stress Natural extracts Antioxidants Physical exercise The prevalence of obesity increases alarmingly every year mostly due to external factors such as high-fat and high-refined sugar intake associated with a sedentary lifestyle. It triggers metabolic disorders such as insulin resistance, hyperlipemia, non-alcoholic fatty liver disease, chronic inflammation, oxidative stress, and gut microbiota dysbiosis. The aim of this study was to evaluate the beneficial effects of a combined intervention with caloric restriction, nutraceutical intake, and a mixed training protocol on oxidative stress, inflammation, and gut dysbiosis derived from the development of obesity in a C57BL6/J mouse experimental model of diet-induced obesity (4.6 Kcal/g diet, 45% Kcal as fat, and 20% fructose in the drinking fluid). The nutraceutical was formulated with ethanolic extracts of Argania spinosa pulp (10%) and Camelina sativa seeds (10%) and with protein hydrolysates from Psoralea corylifolia seeds (40%) and Spirodela polyrhiza whole plants (40%). The combination of nutraceutical and exercise decreased the animals’ body weights and inflammatory markers (TNFα, IL-6, and resistin) in plasma, while increasing gene expression of cat, sod2, gsta2, and nqo1 in the liver. Obese animals showed lower β-diversity of microbiota and a higher Firmicutes/Bacteroidetes ratio vs. normocaloric controls that were reversed by all interventions implemented. Dietary inclusion of a nutraceutical with high antioxidant potential combined with an exercise protocol can be beneficial for bodyweight control and improvement of metabolic status in patients undergoing obesity treatment. 2024-05-27T10:38:32Z 2024-05-27T10:38:32Z 2024-02-23 journal article Guzmán-Carrasco, A.; Kapravelou, G.; López-Jurado, M.; Bermúdez, F.; Andrés-León, E.; Terrón-Camero, L.C.; Prados, J.; Melguizo, C.; Porres, J.M.; Martínez, R. A Novel Plant-Based Nutraceutical Combined with Exercise Can Revert Oxidative Status in Plasma and Liver in a Diet-Induced-Obesity Animal Model. Antioxidants 2024, 13, 274. https://doi.org/10.3390/antiox 13030274 https://hdl.handle.net/10481/92105 10.3390/antiox13030274 eng http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional