Correlation between the presence of cytomegalovirus antibodies and antigen in blood leukocytes for the diagnosis of primary active infection Gutiérrez Fernández, José Piédrola Angulo, Gonzalo Maroto, C. Background: The diagnosis of cytomegalovirus infections measuring IgG or IgM antibodies has a high rate of false positive or negative results, specially in immunocompromised patients. Aim: To compare the diagnostic yield of antibodies against cytomegalovirus with the measurement of the antigen in peripheral leukocytes. Material and methods: Forty three blood samples coming from pediatric patients with suspected cytomegalovirus infections were analyzed. Low affinity IgG and IgM antibodies against Epstein Barr virus and cytomegalovirus, using indirect ELISA assays, and the virus antigen in peripheral leukocytes, using a commercial immunoperoxidase assay, were measured. Results: Seven patients had positive IgM antibodies against cytomegalovirus. In five of these the viral antigen was detected in peripheral leukocytes. Twenty patients had positive antibodies against Epstein Barr virus, and in 16 patients all serologic tests were negative. Conclusions: There is not a good correlation between antibodies against cytomegalovirus and the detection of its antigen in patients with acute infections. 2024-05-15T11:10:56Z 2024-05-15T11:10:56Z 1998-05 journal article Gutiérrez J, Piédrola G, Maroto C. Correlación entre la presencia de anticuerpos y antígeno de citomegalovirus en los leucocitos de sangre para el diagnóstico de la infección activa primaria [Correlation between the presence of cytomegalovirus antibodies and antigen in blood leukocytes for the diagnosis of primary active infection]. Rev Med Chil. 1998 May;126(5):533-7. Spanish. PMID: 9731435 https://hdl.handle.net/10481/91827 spa http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Rev Med Chil