Characterization of daptomycin non-susceptible Enterococcus faecium producing urinary tract infection in a renal transplant recipient Solorzano Puerto, Antonio Panesso, Diana Navarro-Marí, José María Arias, Cesar A Gutiérrez Fernández, José Objectives: Characterization of a urine isolate of daptomycin non-susceptible Enterococcus faecium recovered from a patient with kidney transplantation and no history of daptomycin exposure. Methods: After isolation in a urine sample, identification of E. faecium was confirmed by amplification of the E. faecium-specific gene encoding D-alanyl-D-alanine ligase (ddl) and daptomycin susceptibility testing was performed by E-test on cation-adjusted Mueller-Hinton agar. In order to determine the genetic bases of daptomycin resistance, the open reading frames of five genes previously associated with daptomycin resistance in enterococci were sequenced. Results: Substitutions in the response regulator LiaR (S19F) and cardiolipin synthase (R218Q) were identified. Conclusions: To the best of our knowledge, this is the first characterization of emerging daptomycin resistance in E. faecium in a Spanish hospital in the absence of daptomycin exposure and in a renal transplant recipient. 2024-05-15T10:13:40Z 2024-05-15T10:13:40Z 2015-08 journal article Sorlózano A, Panesso D, Navarro-Marí JM, Arias CA, Gutiérrez-Fernández J. Characterization of daptomycin non-susceptible Enterococcus faecium producing urinary tract infection in a renal transplant recipient. Rev Esp Quimioter. 2015 Aug;28(4):207-9. PMID: 26200029 https://hdl.handle.net/10481/91815 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Rev Esp Quimioter .