Binge drinking leads to an oxidative and metabolic imbalance in skeletal muscle during adolescence in rats: endocrine repercussion Romero-Herrera, Inés Nogales, Fátima Díaz Castro, Javier Moreno Fernández, Jorge Gallego‑Lopez, María del Carmen Ochoa Herrera, Julio José Carreras, Olimpia Ojeda, María Luisa Binge drinking Adolescence Skeletal muscle Supplementary Information The online version contains supplementary material available at https://doi.org/10.1007/s13105-023-00983-z. Binge drinking (BD) is an especially pro-oxidant model of alcohol consumption, mainly used by adolescents. It has recently been related to the hepatic IR-process. Skeletal muscle is known to be involved in insulin action and modulation through myokine secretion. However, there is no information on muscle metabolism and myokine secretion after BD exposure in adolescents. Two experimental groups of adolescent rats have been used: control and BD-exposed one. Oxidative balance, energy status and lipid, and protein metabolism have been analyzed in muscle, together with myokine serum levels (IL-6, myostatin, LIF, IL-5, fractalkine, FGF21, irisin, BDNF, FSTL1, apelin, FABP3, osteocrin, osteonectin (SPARC), and oncostatin). In muscle, BD affects the antioxidant enzyme balance leading to lipid and protein oxidation. Besides, it also increases the activation of AMPK and thus contributes to decrease SREBP1 and pmTOR and to increase FOXO3a expressions, promoting lipid and protein degradation. These alterations deeply affect the myokine secretion pattern. This is the first study to examine a general myokine response after exposure to BD. BD not only caused a detrimental imbalance in myokines related to muscle turnover, decreased those contributing to increase IR-process, decreased FST-1 and apelin and their cardioprotective function but also reduced the neuroprotective BDNF. Consequently, BD leads to an important metabolic and energetic disequilibrium in skeletal muscle, which contributes to exacerbate a general IR-process. 2024-05-14T10:00:10Z 2024-05-14T10:00:10Z 2023-09-07 journal article Romero-Herrera, I., Nogales, F., Diaz-Castro, J. et al. Binge drinking leads to an oxidative and metabolic imbalance in skeletal muscle during adolescence in rats: endocrine repercussion. J Physiol Biochem 79, 799–810 (2023). [https://doi.org/10.1007/s13105-023-00983-z] https://hdl.handle.net/10481/91750 10.1007/s13105-023-00983-z eng http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional Springer Nature