Characterization of daptomycin non-susceptible Enterococcus faecium producing urinary tract infection in a renal transplant recipient Sorlozano Puerto, Antonio Panesso, Diana Navarro-Marí, José María Arias, César A. Gutiérrez Fernández, José Enterococcus faecium Daptomycin non-susceptible LiaR Cardiolipin synthase Daptomicina Cardiolipina sintetasa This work was supported only by the National Institute of Allergy and Infectious Diseases (National Institutes of Health [NIH] grant R01 AI093749 to C. A. A.) Objectives: Characterization of a urine isolate of daptomycin non-susceptible Enterococcus faecium recovered from a patient with kidney transplantation and no history of daptomycin exposure. Methods: After isolation in a urine sample, identification of E. faecium was confirmed by amplification of the E. faecium-specific gene encoding D-alanyl-D-alanine ligase (ddl) and daptomycin susceptibility testing was performed by E-test on cation-adjusted Mueller-Hinton agar. In order to determine the genetic bases of daptomycin resistance, the open reading frames of five genes previously associated with daptomycin resistance in enterococci were sequenced. Results: Substitutions in the response regulator LiaR (S19F) and cardiolipin synthase (R218Q) were identified. Conclusions: To the best of our knowledge, this is the first characterization of emerging daptomycin resistance in E. faecium in a Spanish hospital in the absence of daptomycin exposure and in a renal transplant recipient. Objetivos. Presentamos la caracterización de un aislado de Enterococcus faecium no sensible a daptomicina, recuperado de una muestra de orina de un paciente con trasplante de riñón e infección urinaria y sin antecedentes de exposición previa a daptomicina. Métodos. Tras el aislamiento, la identificación de E. faecium fue confirmada por la amplificación del gen que codifica la región específica de la ligasa de la D-alanil-D-alanina (ddl) y la prueba de sensibilidad a daptomicina se realizó mediante E-test en agar Mueller-Hinton ajustado para cationes. Con el fin de determinar las bases genéticas de la resistencia a daptomicina, se secuenciaron las regiones de lectura abierta de cinco genes previamente asociados con la resistencia a daptomicina en enterococos. Resultados. Se identificaron cambios en el promotor de LiaR (S19F) y la sintetasa de la cardiolipina (R218Q). Conclusiones. Esta es la primera caracterización de un aislado clínico de E. faecium con resistencia a daptomicina en un hospital español, en ausencia de exposición previa y en un receptor de trasplante renal. 2024-05-09T11:54:51Z 2024-05-09T11:54:51Z 2015-08 journal article Sorlózano A, Panesso D, Navarro-Marí JM, Arias CA, Gutiérrez-Fernández J. Characterization of daptomycin non-susceptible Enterococcus faecium producing urinary tract infection in a renal transplant recipient. Rev Esp Quimioter. 2015 Aug;28(4):207-9 PMID: 26200029 https://hdl.handle.net/10481/91595 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Sociedad Española de Quimioterapia