Crystal Structure of the Apo and the ADP-Bound Form of Choline Kinase from Plasmodium falciparum Torretta, Archimede López Cara, Luisa Carlota Parisini, Emilio Among the malaria-causing parasites, the deadliest is Plasmodium falciparum, which accounts for the majority of the fatalities. As the infection progresses inside erythrocytes, major cellular and metabolic changes take place. For its own growth, the parasite relies on the accumulation of phospholipids, which are essential for membrane synthesis. Within the Kennedy pathway, the P. falciparum choline kinase (PfChoK) has a central role in the biosynthesis of phosphatidylcholine and its selective inhibition leads to the parasite arrest and eradication. Here, we report the crystal structure of the apo and the ADP-bound form of choline kinase from Plasmodium falciparum at 2.0 and 2.2 Å resolution, respectively. These new structural data will facilitate the implementation of effective structure-based drug development strategies against PfChoK in the fight against malaria. 2024-04-30T08:59:20Z 2024-04-30T08:59:20Z 2020-07-08 journal article Torretta, A.; Lopez-Cara, L.C.; Parisini, E. Crystal Structure of the Apo and the ADP-Bound Form of Choline Kinase from Plasmodium falciparum. Crystals 2020, 10, 613. https://doi.org/10.3390/cryst10070613 https://hdl.handle.net/10481/91274 https://doi.org/10.3390/cryst10070613 eng http://creativecommons.org/licenses/by-nc-nd/3.0/ open access Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License MDPI