Changes in D1 but not D2 dopamine or mu-opioid receptor expression 4 in limbic and motor structures after lateral hypothalamus electrical 5 self-stimulation: A quantitative autoradiographic study Simón Ferre, María José Puerto Salgado, Amadeo Intracranial self-stimulation (ICSS) Appetitive motivation Dopamine Opioids Quantitative autoradiography Rats This work was supported by grants to Dr. A. Puerto from the Ministry of Education and Science (National R + D Plan BSO2003-06627, PSI2010-17400) and by grants to Dr. E. Ambrosio from the Ministry of Health, Social Services and Equality (Addictive Disorder Network [RD12/0028/0020] of the Carlos III Health Institute); National Drugs (Plan 2012I0057) and the National Distance Education University (Research Promotion Plan). Part of this study was carried out in the Department of Psychobiology of the National Distance Education University (Madrid, Spain) and supported by funds to Dr. Maria J. Simon from the University of Granada and Regional Government of Andalusia (Stays of Excellence). Intracranial self-stimulation (ICSS) of the lateral hypothalamus (LH) is involved in the activation of neuroanatomical systems that are also associated with the processing of natural and other artificial rewarding stimuli. Specific components of this behavior (hedonic impact, learning, and motor behavior) may involve changes in different neurotransmitters, such as dopamine and opioids. In this study, quantitative autoradiography was used to examine changes in muopioid and D1/D2-dopamine receptor expression in various anatomical regions related to the motor and mesolimbic reward systems after intracranial self-stimulation of the LH. Results of the behavioral procedure and subsequent radiochemical assays show selective changes in D1 but not D2 or mu receptors in Accumbens-Shell, Ventral Pallidum, Caudate–Putamen, and Medial Globus Pallidus. These findings are discussed in relation to the different psychobiological components of the appetitive motivational system, identifying some dissociation among them, particularly with respect to the involvement of the D1-dopamine subsystem (but not D2 or mu receptors) in goal-directed behaviors. 2024-04-29T07:15:38Z 2024-04-29T07:15:38Z 2015-11-30 info:eu-repo/semantics/article Published version: Simón Ferre, María José et al. Changes in D1 but not D2 dopamine or mu-opioid receptor expression in limbic and motor structures after lateral hypothalamus electrical self-stimulation: A quantitative autoradiographic study. Neurobiology of Learning and Memory Volume 127, January 2016, Pages 17-26. https://doi.org/10.1016/j.nlm.2015.11.007 https://hdl.handle.net/10481/91224 10.1016/j.nlm.2015.11.007 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional Elsevier