Effect of Punicalagin and Ellagic Acid on Human Fibroblasts In Vitro: A Preliminary Evaluation of Their Therapeutic Potential Illescas Montes, Rebeca Rueda-Fernández, Manuel González Acedo, Anabel Melguizo Rodríguez, Lucía Raquel García Recio, Enrique Ramos Torrecillas, Javier García Martínez, Olga Pomegranate Phenolic compounds Punicalagin Background: Pomegranate is a fruit that contains various phenolic compounds, including punicalagin and ellagic acid, which have been attributed to anti-inflammatory, antioxidant, and anticarcinogenic properties, among others. Objective: To evaluate the effect of punicalagin and ellagic acid on the viability, migration, cell cycle, and antigenic profile of cultured human fibroblasts (CCD-1064Sk). MTT spectrophotometry was carried out to determine cell viability, cell culture inserts were used for migration trials, and flow cytometry was performed for antigenic profile and cell cycle analyses. Cells were treated with each phenolic compound for 24 h at doses of 10−5 to 10−9 M. Results: Cell viability was always significantly higher in treated versus control cells except for punicalagin at 10−9 M. Doses of punicalagin and ellagic acid in subsequent assays were 10−6 M or 10−7 M, which increased the cell migration capacity and upregulated fibronectin and α-actin expression without altering the cell cycle. Conclusions: These in vitro findings indicate that punicalagin and ellagic acid promote fibroblast functions that are involved in epithelial tissue healing. 2024-04-28T16:39:39Z 2024-04-28T16:39:39Z 2023-12-20 journal article Illescas-Montes, R.; Rueda-Fernández, M.; González-Acedo, A.; Melguizo-Rodríguez, L.; García-Recio, E.; Ramos-Torrecillas, J.; García-Martínez, O. Effect of Punicalagin and Ellagic Acid on Human Fibroblasts In Vitro: A Preliminary Evaluation of Their Therapeutic Potential. Nutrients 2024, 16, 23. https://doi.org/10.3390/nu16010023 https://hdl.handle.net/10481/91197 10.3390/nu16010023 eng http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional MDPI