Metal-dependent Glycosylation in Recombinant Metallothioneins García Risco, Mario González Garnica, Ana Isabel Calatayud, Sara López Jaramillo, Francisco Javier Pedrini Martha, Veronika Albalat, Ricard Dallinger, Reinhard Domínguez Vera, José Manuel Palacios, Óscar Capdevila, Mercè Researchers from Barcelona and Granada want to acknowledge the Spanish Ministerio de Ciencia e Innovación and FEDER for the projects BIO2015-67358-C2-2-P (M.C. and Ò.P.), PID2021-123258NB-I00 (R.A.) and PID2019-111461GB-I00 (J.M.D.V). This study was co-financed by a cooperation grant to R.D. from the Austrian Science Fund (DACH grant No I 3032-B21). M.C. and Ò.P. are members of the “Grup de Recerca de la Generalitat de Catalunya”, ref. 2017SGR-864, and R.A. of ref. 2017SGR-1665. M.G.-R. acknowledges to the UAB the PIF grant. A.G. acknowledges Junta de Andalucía for the postdoctoral contract within the PAIDI 2020 program (DOC_00791). We thank the Servei d’Anàlisi Química (SAQ) at the Universitat Autònoma de Barcelona (ICP-AES, ESI-MS) for allocating instrument time. R.D. and V.P.-M. are members of the Center of Molecular Biosciences of the University of Innsbruck. We show for the first time glycosylation of recombinant metallothioneins (MTs) produced in E. coli. Interestingly, our results show that the glycosylation level of the recombinant MTs is inversely proportional to the degree of protein structuration, and reflects their different metal preferences. 2024-04-04T07:17:49Z 2024-04-04T07:17:49Z 2022-11-15 journal article Published version: García Risco, Mario et al. Metal-dependent Glycosylation in Recombinant Metallothioneins. Chem. Commun., 2022,58, 13755-13758. https://doi.org/10.1039/D2CC05589A https://hdl.handle.net/10481/90378 10.1039/D2CC05589A eng open access Royal Society of Chemistry