Detergent-based decellularized peripheral nerve allografts: An in vivo preclinical study in the rat sciatic nerve injury model Chato Astrain, Jesús Philips, Charlot Campos Sánchez, Fernando Durand Herrera, Daniel García García, Óscar Darío Roosens, Annelies Alaminos Mingorance, Miguel Campos Muñoz, Antonio Jesús Carriel Araya, Víctor Chemical decellularization Functional recovery Histology Nerve repair Peripheral nerve regeneration Tissue engineering Este estudio ha sido financiado por el "Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica, Ministerio de Economía y Competitividad (Instituto de Salud Carlos III), y cofinanciado por el "Fondo Europeo de Desarrollo Regional (FEDER) Unión Europea", Subvención nº FIS PI17-0393, y por el "Programa Operativo FEDER Andalucía 2014-2020, I +D +I, Frontera" Subvención nº A-CTS-488-UGR18. Todo el estudio se realizó en las instalaciones del Grupo de Ingeniería Tisular (CTS-115) de la Universidad de Granada y en la Unidad Experimental del Hospital Universitario Virgen de las Nieves de Granada, España. Este estudio forma parte de la Tesis Doctoral de Jesús Chato-Astrain. Nerve autograft is the gold standard technique to repair critical nerve defects, but efficient alternatives are needed. The present study evaluated the suitability of our novel Roosens-based (RSN) decellularized peripheral nerve allografts (DPNAs) in the repair of 10-mm sciatic nerve defect in rats at the functional and histological levels after 12 weeks. These DPNAs were compared with the autograft technique (AUTO) and Sondell (SD) or Hudson (HD) based DPNAs. Clinical and functional assessments demonstrated a partial regeneration in all operated animals. RSN-based DPNAs results were comparable with SD and HD groups and closely comparable with the AUTO group without significant differences (p > .05). Overall hematological studies confirmed the biocompatibility of grafted DPNAs. In addition, biochemistry revealed some signs of muscle affection in all operated animals. These results were confirmed by the loss of weight and volume of the muscle and by muscle histology, especially in DPNAs. Histology of repaired nerves confirmed an active nerve tissue regeneration and partial myelination along with the implanted grafts, being the results obtained with HD and RSN-based DPNAs comparable with the AUTO group. Finally, this in vivo study suggests that our novel RSN-based DPNAs supported a comparable tissue regeneration, along the 10-mm nerve gap, after 12-week follow-up to HD DPNAs, and both were superior to SD group and closely comparable with autograft technique. However, further improvements are needed to overcome the efficacy of the nerve autograft technique. 2024-03-20T13:11:00Z 2024-03-20T13:11:00Z 2020-02 journal article Chato-Astrain J, Philips C, Campos F, et al. Detergent-based decellularized peripheral nerve allografts: An in vivo preclinical study in the rat sciatic nerve injury model. J Tissue Eng Regen Med. 2020;14:789–806. https://doi.org/10.1002/term.3043 https://hdl.handle.net/10481/90117 10.1002/term.3043 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Wiley