Sysmex UF-1000i performance for screening yeasts in urine Gutiérrez Fernández, José Riazzo, Cristina Sara Sanbonmatsu, Sara Luna Del Castillo, Juan De Dios Sorlozano Puerto, Antonio Miranda, Consuelo Navarro Marí, José María Urine Automated system Screening Yeast Candida albicans We tested the capacity of the Sysmex UF-1000i system to detect yeasts in urine by screening a total of 22 132 urine samples received for culture in our microbiology laboratory during 1 year. We also analyzed different dilutions of previously filtered urine inoculated with a strain of Candida albicans. With clinical samples, a single cut-off point of 50 yeast-like cells (YLCs)/μL detected candiduria ≥10 000 colony forming units (CFU)/mL and >100 000 CFU/mL with a sensitivity of 87.3%/95.4%, a specificity of 97%, a negative predictive value of 95.9%, and a positive predictive value of 9.3%/5.7%. With the simulated samples, a linear relationship was observed between the dilution factor and the number of cells detected by UF-1000i. This instrument appears to be able to reliably rule out candiduria of a magnitude of at least 10 000 CFU/mL and facilitate urine sample screening, thereby providing fast results. The Sysmex UF1000i system can be adapted for candiduria screening by the use of an appropriate YLCs/μL cut-off point that takes account of the prevalence of candiduria in the population. 2024-03-18T11:14:00Z 2024-03-18T11:14:00Z 2014-04 info:eu-repo/semantics/article Gutiérrez-Fernández J, Riazzo C, Sanbonmatsu S, de Dios Luna J, Sorlózano A, Miranda C, Navarro JM. Sysmex UF-1000i performance for screening yeasts in urine. APMIS. 2014 Apr;122(4):324-8. https://doi.org/10.1111/apm.12148 https://hdl.handle.net/10481/90075 10.1111/apm.12148 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional Wiley