Acceptable performance of VITEK 2 system to detect extended-spectrum β-lactamases in clinical isolates of Escherichia coli: a comparative study of phenotypic commercial methods and NCCLS guidelinesommercial methods and NCCLS guidelines Solorzano Puerto, Antonio Gutiérrez Fernández, José Piédrola Angulo, Gonzalo Soto, María José Escherichia coli ESBL Etest VITEK 2 The disk approximation method, Etest (AB Biodisk, Solna, Sweden), and VITEK 2 system (bioMérieux, Marcy l'Etoile, France) were used to study 399 extended-spectrum beta-lactamase (ESBL)-producing (115 strains) and non-ESBL-producing (284 strains) clinical isolates of Escherichia coli after recommended procedures. Comparative study of the phenotypic findings yielded data on the sensitivity, specificity, and positive and negative predictive values and performance of each method. The sensitivity (100% using 2 substrates), specificity (99.3-100%), and predictive values of the disk approximation, Etest, and VITEK 2 methods were similar. 2024-03-15T11:54:13Z 2024-03-15T11:54:13Z 2005-03 journal article Sorlózano A, Gutiérrez J, Piédrola G, Soto MJ. Acceptable performance of VITEK 2 system to detect extended-spectrum beta-lactamases in clinical isolates of Escherichia coli: a comparative study of phenotypic commercial methods and NCCLS guidelines. Diagn Microbiol Infect Dis. 2005 Mar;51(3):191-3. https://doi.org/10.1016/j.diagmicrobio.2004.11.001 https://hdl.handle.net/10481/90021 10.1016/j.diagmicrobio.2004.11.001 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Elsevier