Prevalence and genetic diversity of Trichomonas vaginalis in the general population of Granada and co-infections with Gardnerella vaginalis and Candida species Carrillo-Ávila, José Antonio Serrano García, María Luisa Fernández Parra, Jorge Sorlozano Puerto, Antonio Navarro Marí, José María Stensvold, C. Rune Gutiérrez Fernández, José Vaginitis MLST Trichomonas vaginalis Gardnerella vaginalis Candida Purpose: Purulent or exudative genitourinary infections are a frequent cause of consultation in primary and specialized healthcare. The objectives of this study were: to determine the prevalence of Trichomonas vaginalis and co-infections with Candida spp. and Gardnerella vaginalis in vaginal secretion; and to use multilocus sequence typing (MLST) to analyse the genetic diversity of T. vaginalis strains. Methodology: The samples were submitted for analysis (n=5230) to a third-level hospital in Granada (Southern Spain) between 2011 and 2014; eight T. vaginalis strains isolated during 2015 were randomly selected for MLST analysis. Culture and nucleic acid hybridization techniques were used to detect microorganisms in the samples. Results: The prevalence of T. vaginalis was 2.4 % between 2011 and 2014, being higher during the first few months of both 2011 and 2012. Among samples positive for T. vaginalis, co-infection with G. vaginalis was detected in 29 samples and co-infection with Candida spp. in 6, while co-infection with all three pathogens was observed in 3 samples. The only statistically significant between-year difference in co-infection rates was observed for T. vaginalis with G. vaginalis due to an elevated rate in 2011. MLST analysis results demonstrated a high genetic variability among strains circulating in our setting. Conclusion: These findings emphasize the need for the routine application of diagnostic procedures to avoid the spread of this sexually transmitted infection. 2024-03-13T12:39:55Z 2024-03-13T12:39:55Z 2017-10 info:eu-repo/semantics/article Carrillo-Ávila et al. Prevalence and genetic diversity of Trichomonas vaginalis in the general population of Granada and co-infections with Gardnerella vaginalis and Candida species. Journal of Medical Microbiology 2017;66:1436–1442. DOI: 10.1099/jmm.0.000603 https://hdl.handle.net/10481/89964 10.1099/jmm.0.000603 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional Microbiology Society