PCR-free and chemistry-based technology for miR-21 rapid detection directly from tumour cells Delgado-Gonzalez, Antonio Robles Remacho, Agustín Marín Romero, Antonio De Miguel Pérez, Diego Guardia Monteagudo, Juan José Fara, Mario A. Sánchez Martín, Rosario María Díaz Mochón, Juan José miRNAs play pivotal roles in various biological contexts, particularly in disease. Notably, miRNA-21 is implicated in severe pathological conditions, necessitating accurate quantification for medical diagnostics. Existing miRNA quantification techniques often fall short. Here, we introduce a novel, dynamic chemistry-based, PCR-free method for rapid and cost-effective miRNA-21 identification and quantification directly from tumor cells, employing FACS and fluorescent microplate assays. This approach, utilizing bead-based reagents, enables precise quantification of miR-21 molecules in MDA-MB-468 and H1975 tumor cells. 2024-02-07T12:07:11Z 2024-02-07T12:07:11Z 2019-08-01 info:eu-repo/semantics/article Delgado-Gonzalez, A. , Robles-Remacho, A. , Marin-Romero, A. , ... Sanchez-Martin, R.M. , Diaz-Mochon, J.J. Talanta, 2019, 200, pp. 51–56 https://hdl.handle.net/10481/88591 https://doi.org/10.1016/j.talanta.2019.03.039 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/embargoedAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional Elsevier