Drug "Clicking" on Cell-Penetrating Fluorescent Nanoparticles for In Cellulo Chemical Proteomics Sánchez Martín, Rosario María This paper introduces a novel method for identifying molecular targets of drugs by conjugating azide-labeled drugs with alkyne-tagged cell-penetrating fluorescent nanoparticles using click chemistry. A modified Baeyer test for alkynes monitors the Cu(I)-catalyzed azide–alkyne cycloaddition reaction, ensuring drug presence on solid support. Dasatinib-conjugated nanoparticles effectively inhibit the SRC protein target in vitro and demonstrate intracellular colocalization. 2024-02-07T10:17:47Z 2024-02-07T10:17:47Z 2018-08-18 journal article Valero T, Delgado-González A, Unciti-Broceta J D, Cano-Cortés V, Pérez-López A M , Unciti-Broceta A, Sánchez Martín R M. Bioconjugate Chem. 2018, 29, 9, 3154–3160, https://doi.org/10.1021/acs.bioconjchem.8b00481 https://hdl.handle.net/10481/88536 doi.org/10.1021/acs.bioconjchem.8b00481 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional ACS Publications