Probiotics Prevent Dysbiosis and the Rise in Blood Pressure in Genetic Hypertension: Role of Short-Chain Fatty Acids Robles Vera, Iñaki Toral Jiménez, Marta Visitación Pastor, Néstor de la Sánchez Santos, Manuel Gómez Guzmán, Manuel Romero Pérez, Miguel Yang, Tao Izquierdo-García, José L Jiménez Moleón, Rosario Ruíz-Cabello, Jesús Guerra Hernández, Eduardo Jesús Raizada, Mohan K Pérez-Vizcaíno, Francisco Duarte Pérez, Juan Manuel Endothelial dysfunction Gut microbiota Hypertension https://pubmed.ncbi.nlm.nih.gov/31953983/ https://onlinelibrary.wiley.com/doi/10.1002/mnfr.201900616 https://onlinelibrary.wiley.com/doi/epdf/10.1002/mnfr.201900616 The objective of this study is to determine the cardiovascular effects of the probiotics Bifidobacterium breve CECT7263 (BFM) and Lactobacillus fermentum CECT5716 (LC40), and the short chain fatty acids butyrate, and acetate in spontaneously hypertensive rats (SHR). Ten five-week old Wistar Kyoto rats (WKY) and fifty aged-matched SHR are randomly distributed into six groups: control WKY, control SHR, treated SHR-LC40, treated SHR-BMF, treated SHR-butyrate, and treated SHR-acetate. Chronic treatments with LC40 or BFM increase butyrate-producing bacteria and prevent the blood pressure increase in SHR. Oral treatment with butyrate or acetate also prevents the increase in both blood pressure and Firmicutes/Bacteroidetes (F/B) ratio. All treatments restore the Th17/Treg balance in mesenteric lymph nodes, normalized endotoxemia, and prevent the impairment of endothelium-dependent relaxation to acetylcholine, as a result of reduced NADPH oxidase-driven reactive oxygen species production. These protective effects might be mediated by both the reduction in vascular lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4) pathway and the increase in Treg infiltration in the vasculature. The probiotics LC40 and BFM prevent dysbiosis and the development of endothelial dysfunction and high blood pressure in genetic hypertension. These effects seem to be related to endotoxemia reduction and to increase Treg accumulation in the vasculature. 2024-01-29T11:37:30Z 2024-01-29T11:37:30Z 2020-03 journal article Robles-Vera I, Toral M, de la Visitación N, Sánchez M, Gómez-Guzmán M, Romero M, Yang T, Izquierdo-Garcia JL, Jiménez R, Ruiz-Cabello J, Guerra-Hernández E, Raizada MK, Pérez-Vizcaíno F, Duarte J. Probiotics Prevent Dysbiosis and the Rise in Blood Pressure in Genetic Hypertension: Role of Short-Chain Fatty Acids. Mol Nutr Food Res. 2020 Mar;64(6):e1900616. doi: 10.1002/mnfr.201900616. Epub 2020 Feb 6. PMID: 31953983. 1613-4125 PMID: 31953983 https://hdl.handle.net/10481/87490 10.1002/mnfr.201900616 eng http://creativecommons.org/licenses/by-nc-nd/3.0/ embargoed access Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim