Antibiofilm Activity of Diclofenac and Antibiotic Solutions in Endodontic Therapy Ferrer Luque, Carmen María Baca García, María Pilar Solana, Carmen Rodríguez Archilla, Alberto Arias Moliz, María Teresa Ruiz Linares, Matilde Introduction: The aim of this study was to compare the antibiofilm effects of a triple antibiotic solution (TAS); a double antibiotic solution (DAS); and 5%, 2.5%, and 1.25% diclofenac solutions (DCSs) against Enteroccocus faecalis biofilm. Methods: Eighty-four sterile radicular dentin blocks were used as biofilm substrate for 3 weeks. The study groups were as follows: (1) 1 mg/mL TAS (minocycline, metronidazole, and ciprofloxacin), (2) 1 mg/mL DAS (metronidazole and ciprofloxacin), (3) 5% DCS, (4) 2.5% DCS, (5) 1.25% DCS, and (6) 0.9% saline solution. The antimicrobial activity was evaluated by bacterial count determinations and confocal laser scanning microscopy. The contact time for the antimicrobial tests was 5 minutes. Bacterial counts were expressed as the reduction percentage of colony-forming units; for the confocal laser scanning microscopic evaluation, the log10 total biovolume and percentage of green population (live cells) were calculated. Results: The colony-forming unit reduction percentage ranged between 62.98 and 98.62, respectively, for TAS and 5% DCS. The DCS showed a concentration-dependent effect.For the confocal laser scanning microscopy, the log10 total biovolume in all groups was very similar and showed a scarce (1.39-1.02) but significant reduction with respect to the control; 5% and 2.5% DCSs gave the lowest viable cell percentage. The TAS and DAS groups showed intermediate values without significant differences between them. Conclusions: DCSs at 5% and 2.5% have greater antimicrobial effects than TAS and DAS and may be considered a valid alternative for controlling the infection of teeth with apical periodontitis. 2024-01-26T11:48:17Z 2024-01-26T11:48:17Z 2021-04-20 journal article Ferrer-Luque CM, Baca P, Solana C, Rodríguez-Archilla A, Arias-Moliz MT, Ruiz-Linares M. Antibiofilm Activity of Diclofenac and Antibiotic Solutions in Endodontic Therapy. J Endod. 2021 Jul;47(7):1138-1143 https://hdl.handle.net/10481/87368 10.1016/j.joen.2021.04.004 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Elsevier