Effect of olive oil phenolic compounds on osteoblast differentiation Melguizo Rodríguez, Lucía Raquel Manzano Moreno, Francisco Javier Luna Bertos, María Elvira De Rivas Velasco, Ana María Ramos Torrecillas, Javier Ruiz Rodríguez, Concepción García Martínez, Olga "This is the peer reviewed version of the following article: Melguizo-Rodríguez L., Manzano-Moreno F.J., De Luna-Bertos E., Rivas A., Ramos-Torrecillas J., Ruiz C., García-Martínez O. Effect of olive oil phenolic compounds on osteoblast differentiation. European Journal of Clinical Investigation, 2018; 48(4): 1-6, which has been published in final form at 10.1111/eci.12904. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited." Background: Osteoporosis is a skeletal disorder characterized by compromised bone strength that predisposes individuals to an increased risk of fracture. Previous in vivo and in vitro studies have reported that phenolic compounds present in extra virgin olive oil have a beneficial effect on osteoblasts in terms of increase cell proliferation. The aim of this study was to determine whether phenolic compounds present in olive oil could modify the expression of cell differentiation markers on osteoblasts. Study Design: An in vitro experimental design was peformed using MG-63 osteoblasts cell line. Methods: MG63 cells were exposed to different doses of luteolin, apigenin, or p-coumaric, caffeic, or ferulic acid. Alkaline phosphatase (ALP) was evaluated by spectrophotometry and antigen expression (CD54, CD80, CD86, and HLA-DR) by flow cytometry. Results: At 24 h, treated groups showed an increased ALP and modulated antigen profile, with respect to the non-treated group. Conclusion: These results demonstrate that the phenolic compounds studied induce cell maturation in vitro, increasing ALP synthesis and reducing the expression of antigens involved in immune functions of the osteoblast which would improve bone density. 2024-01-26T11:27:56Z 2024-01-26T11:27:56Z 2018 journal article Published version: Melguizo-Rodríguez L, Manzano-Moreno FJ, De Luna-Bertos E, et al. Effectof olive oil phenolic compounds on osteoblastdifferentiation.Eur J Clin Invest. 2018;48:e12904. DOI: 10.1111/eci.12904 https://hdl.handle.net/10481/87362 10.1111/eci.12904 eng open access