Optical molecular imaging of lysyl oxidase activity– detection of active fibrogenesis in human lung tissue Aslam, Tashfeen Miele, Amy Chankeshwara, Sunay V. Megía Fernández, Alicia Michels, Chesney Akram, Ahsan R. McDonald, Neil Hirani, Nik Haslett, Chris Bradley, Mark Dhaliwal, Kevin LOX Optical imaging Fluorescence Aberrant fibrogenesis is a feature of many diseases in multiple organ systems. The lysyl oxidase family of enzymes are central to tissue homeostasis and elevated lysyl oxidase activity is implicated in fibroproliferation as well as in cancer stroma. We have synthesised a novel fluorogenic reporter for monitoring lysyl oxidase activity that generates a 3–5 fold increase in fluorescence following probe activation in ventilating fibrotic ex vivo asinine lung and ex vivo human lung tissue. The probe termed “oLOX” can provide real-time measurement of lysyl oxidase activity in a number of biological settings and is tractable from an in vitro setting to man 2024-01-26T08:43:32Z 2024-01-26T08:43:32Z 2015 journal article Chem. Sci., 2015, 6, 4946–4953 https://hdl.handle.net/10481/87331 10.1039/c5sc01258a eng Programme FP7 2012 under grant agreement no. 326465 (A.M-F.) info:eu-repo/grantAgreement/EC/FP7/326465 http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Royal Society of Chemistry