Medicago sativa L.: mejora y nuevos aspectos de su valor nutritivo y funcional por co-inoculación bacteriana Martínez Martínez, Rosario Nebot Valenzuela, Elena Porres Foulquie, Jesús María Kapravelou, Garyfallia Moral García, Ana Isabel Del Talbi, Chouhra Bedmar Gómez, Eulogio José López-Jurado Romero De La Cruz, María Objective: to study the effect of co-inoculation with Ensifer meliloti and Halomonas maura of the leguminous Medicago sativa L., on growth, nutritional and functional value, grown under salinity conditions. Methods: plants of M. sativa were grown in a solution with a mixture of salts (CaSO4, MgCl, NaCl and NaHCO 3) and were co-inoculated with its specific rhizobium and the halophilic moderated bacterium H. maura. Different physiologic parameters were determined, as well as, nitrogen and minerals content. Furthermore, an assay of in vitro digestibility was carried out. Results: salinity had a negative effect on the plants; however, co-inoculation increased yield, nitrogen content, total minerals, Ca and Mg. Moreover, physiologic parameters as water potential and leghemoglobin content in fresh nodules were higher compared to those of plants inoculated only with E. meliloti. Both, salinity and bacterial treatment with E. meliloti and H. maura increased the antioxidant capacity of the legume, in dialyzates and retentates collected after an in vitro digestibility assay. Conclusion: co-inoculation of plants with E. meliloti and H. maura could improve the alfalfa yield under specific salinity conditions, increasing the nutritional and functional value of the plants. M. sativa could be considered in the formulations of nutritional supplements for the human diet. 2024-01-24T11:15:22Z 2024-01-24T11:15:22Z 2015 journal article Rosario Martínez, Elena Nebot, Jesús María Porres, Garyfallia Kapravelou, Ana del Moral, Chouhra Talbi, Eulogio José Bedmar, María López-Jurado. Medicago sativa L.: mejora y nuevos aspectos de su valor nutritivo y funcional por co-inoculación bacteriana. Nutrición Hospitalaria, 2015;32(6):2741-2748. ISSN 0212-1611. DOI:10.3305/nh.2015.32.6.9849 Area: Nutrition & Dietetics (60/80), Q3, T3. IF: 1.497 (2015). https://hdl.handle.net/10481/87191 10.3305/nh.2015.32.6.9849 spa http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Arán ediciones