Centromeric Satellite DNA in Flatfish (Order Pleuronectiformes) and Its Relation to Speciation Processes Robles Rodríguez, Francisca Herrán Moreno, Roberto De La Navajas Pérez, Rafael Cano-Roldán, Belén Sola-Campoy, Pedro Juan García Zea, Jerson Alexander Ruiz Rejón, Carmelo Centromeric satellite DNA Flatfishes Molecular evolution This work was supported by the Spanish Ministry of Science and Innovation (AGL2009-11872), PLEUROGENE (GEN2003) project funded by the Genome Canada-Genoma España joint programme, and the Consolider-Ingenio AQUAGENOMICS Project (CSD2007-00002). Two new centromeric satellite DNAs in flatfish (Order Pleuronectiformes) have been characterized. The SacI-family from Hippoglossus hippoglossus, restricted to this species, had a monomeric size of 334 base pair (bp) and was located in most of the centromeres of its karyotype. The PvuII-family, with a monomeric size of 177 bp, was initially isolated from the genome of Solea senegalensis, and fluorescent in situ hybridization (FISH) localized the repeat to centromeres of most of the chromosomes. This family could only be amplified in 2 other species of the genus Solea (Solea solea and Solea lascaris). Molecular features and chromosomal location indicated a possible structural and/or functional role of these sequence repeats. The presence of species-specific satellite-DNA families in the centromeres and their possible role in the speciation processes in this group of fishes is discussed. 2024-01-19T11:38:32Z 2024-01-19T11:38:32Z 2017 journal article Rodríguez FR, de la Herrán R, Navajas-Pérez R, Cano-Roldán B, Sola-Campoy PJ, García-Zea JA, Rejón CR. Centromeric Satellite DNA in Flatfish (Order Pleuronectiformes) and Its Relation to Speciation Processes. J Hered. 2017 Mar 1;108(2):217-222. https://hdl.handle.net/10481/86961 10.1093/jhered/esw076 eng http://creativecommons.org/licenses/by-nc-nd/3.0/ embargoed access Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License