Illuminating the human genome Simpson, Jeremy C Neubrand, Veronika Elisabeth Wiemann, Stefan Pepperkok, Rainer cDNA, GFP, Subcellular localisation Supplementary material for this paper can be obtained electronically by using Springer LINK Server located at http://dx.doi.org/10.1007/s004180000236 Acknowledgements The Advanced Light Microscopy Facility at EMBL is supported by BioRad (UK), Bitplane (Switzerland), Eppendorf (Germany), Evotec (Germany), Improvision (UK), Leica (Germany), Nikon (Europe), Olympus (Europe), Perkin Elmer (UK), T.I.L.L. Photonics (Germany) and Zeiss (Germany). The Molecular Genome Analysis Department is supported by the BMBF as part of the German Genome Project. J.C.S. is supported by an EMBO Long Term Fellowship. The identification and analysis of novel genes and their encoded protein products remains a vigorous area of research in biology today. Worldwide genomic and cDNA sequencing projects are now identifying new molecules every day and the need for methodologies to functionally characterise these proteins has never been greater. The distinct compartmental arrangement of eukaryotic cells helps define the processes which occur within or in proximity to these membranes, and as such provides one means of inferring protein function. We describe here some of the methods recently reported in the literature, which use the subcellular localisation of proteins as a first step towards their further characterisation. 2024-01-03T11:14:03Z 2024-01-03T11:14:03Z 2000 journal article Histochem Cell Biol 115: 23–29 https://hdl.handle.net/10481/86539 10.1007/s004180000236 eng http://creativecommons.org/licenses/by-nc-nd/3.0/ embargoed access Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License Springer Nature