Effects of oxytocin administration on the hydromineral balance of median eminence-lesioned rats Mahía, Javier Bernal, Antonio Puerto, Amadeo Cerebral salt wasting Hydromineral regulation Median eminence Natriuresis Oxytocin Plasma sodium depletion Sodium appetite Water intake In the clinical setting, acute injuries in hypothalamic mediobasal regions, along with polydipsia and polyuria, have been observed in patients with cerebral salt wasting (CSW). CSW is also characterised by hypovolaemia and hyponatraemia as a result of an early increase in natriuretic peptide activity. Salt and additional amounts of fluid are the main treatment for this disorder. Similarly, experimental lesions to these brain regions, which include the median eminence (ME), produce a well-documented neurological model of polydipsia and polyuria in rats, which is preceded by an early sodium excretion of unknown cause. In the present study, oxytocin (OT) was used to increase the renal sodium loss and prolong the hydroelectrolyte abnormalities of ME-lesioned animals during the first few hours post-surgery. The objective was to determine whether OT-treated ME-lesioned animals increase their sodium appetite and water intake to restore the volume and composition of extracellular body fluid. Electrolytic lesion of the ME increased water intake, urinary volume and sodium excretion of food-deprived rats and also decreased urine osmolality and estimated plasma sodium concentration. OT administration at 8 hours post-surgery reduced water intake, urine output and plasma sodium concentration and also increased urine osmolality and urine sodium excretion between 8 and 24 hours post-lesion. From 24 to 30 hours, more water and hypertonic NaCl was consumed by OT-treated ME-le- sioned rats than by physiological saline-treated-ME-lesioned animals. Food availabil- ity from 30 to 48 hours reduced the intake of hypertonic saline solution by ME/OT animals, which increased their water and food intake during this period. OT admin- istration therefore appears to enhance the natriuretic effect of ME lesion, produc- ing hydroelectrolyte changes that reduce the water intake of food-deprived animals. Conversely, the presence of hypertonic NaCl increases the fluid intake of these ani- mals, possibly as a result of the plasma sodium depletion and hypovolaemic states previously generated. Finally, the subsequent increase in food intake by ME/OT ani- mals reduces their need for hypertonic NaCl but not water, possibly in response to osmotic thirst. These results are discussed in relation to a possible transient activa- tion of the ME with the consequent secretion of natriuretic peptides stored in termi- nal swellings, which would be augmented by OT administration. Electrolytic lesion of the ME may therefore represent a useful neurobiological model of CSW. 2023-12-19T08:03:04Z 2023-12-19T08:03:04Z 2019-05-26 info:eu-repo/semantics/article Published version: Javier Mahía, Antonio Bernal and Amadeo Puerto. Effects of oxytocin administration on the hydromineral balance of median eminence-lesioned rats. Journal of Neuroendocrinology. doi:10.1111/jne.12778 https://hdl.handle.net/10481/86329 10.1111/jne.12778 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional Wiley