Argan pulp as a novel functional ingredient with beneficial effects on multiple metabolism biomarkers Martínez Martínez, Rosario Ortuño Guzmán, Ana María Kapravelou, Garyfallia Melguizo Alonso, Consolación Prados Salazar, José Carlos López-Jurado Romero De La Cruz, María Porres Foulquie, Jesús María Argan pulp Functional ingredient Polyphenols Insulin resistance Gut microbiota Antioxidant activity Argan tree is mainly used to obtain the oil from its seeds in a process that generates by-products such as the pulp of the fruit. The aim of this study was to assess the in vitro antioxidant activity and profile of bioactive compounds from an argan fruit extract, and to assay the metabolic effects of the argan pulp intake in a mouse experimental model. Our results showed a high in vitro antioxidant activity in ethanolic extracts of argan juice and pulp measured as total polyphenol content, reducing capacity, ABTS, DPPH, and lipid peroxidation inhibition capacity. In addition, several bioactive compounds with antioxidant, hypoglycemic, and anti-inflammatory activity were identified (vaccihein A, bergapten, methyl chloragenate, 2-O-caffeoyl glucaric acid, avicularin, quercetin-3- O-sophoroside, prodelphinidin B4 and mirificin). Dietary intake of argan pulp induced a remarkable hypoglycemic activity and decreased the gene expression of inflammatory markers. Gene expression and enzyme activity of liver antioxidant and detoxifying enzymes were differentially affected by argan pulp intake, whereas gut microbiota was modulated decreasing the Firmicutes/Bacteroidetes ratio. In conclusion, argan pulp can be considered a functional food or ingredient with beneficial effects on several metabolism biomarkers. 2023-11-03T10:28:11Z 2023-11-03T10:28:11Z 2023-10-27 journal article R. Martínez et al. Argan pulp as a novel functional ingredient with beneficial effects on multiple metabolism biomarkers. Journal of Functional Foods 110 (2023) 105864[https://doi.org/10.1016/j.jff.2023.105864] https://hdl.handle.net/10481/85440 10.1016/j.jff.2023.105864 eng http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional Elsevier