Efficacy of mixed diclofenac solutions against root canal biofilms Ferrer Luque, Carmen María Baca García, María Pilar Arias Moliz, María Teresa Ruiz Linares, Matilde Antimicrobial activity Cetrimide Diclofenac Polymicrobial biofilm Root canal dentin Research Group CTS- 167 of the Junta de Andalucia, Spain The objective of this research was to evaluate the efficacy of diclofenac sodium solutions, with or without cetrimide (CTR) added, against polymicrobial root canal biofilms grown in dentin specimens. The study groups were: (1) 5% diclofenac sodium (DCS); (2) 2.5% DCS; (3) 2.5% DCS + 0. 2% CTR; (4) 2.5% DCS + 0.4% CTR and (5) 0.9% saline solution (SS) as the control. After 5 min of solution contact with the biofilms, the antimicrobial activity was evaluated by means of the adenosine triphosphate (ATP) assay as well as confocal laser scanning microscopy (CLSM). Microbial quantification was indicated as the percentage reduction of relative light units (RLUs) for the ATP assay, the Log(10) total biovolume and the viability percentage (green cells) for CLSM. Solutions of 2.5% DCS + 0.4% CTR and 5% DCS showed the highest antimicrobial efficacy. Cetrimide increased the antibiofilm activity of diclofenac sodium against endodontic biofilms. 2023-09-20T12:19:38Z 2023-09-20T12:19:38Z 2023-07-18 journal article Ferrer- Luque CM, Solana C, Aguado B, Baca P, Arias-Moliz MT, Ruiz-Linares M. Efficacy of mixed diclofenac solutions against root canal biofilms. Aust Endod J. 2023;00:1–7. [https://doi.org/10.1111/aej.12776] https://hdl.handle.net/10481/84526 10.1111/aej.12776 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Wiley