Postharvest abscisic acid treatment modulates the primary metabolism and the biosynthesis of t-zeatin and ribofavin in zucchini fruit exposed to chilling stress Castro Cegrí, Alejandro Carvajal, Fátima Osorio, Sonia Jamilena, Manuel Garrido Garrido, Dolores Palma Martín, Francisco José ABA Cucurbita pepo Postharvest Vitamin B2 Cold stress Cytokinins Appendix A. Supporting information Supplementary data associated with this article can be found in the online version at doi:10.1016/j.postharvbio.2023.112457 Abscisic acid (ABA) plays an important role in the regulation of several stress responses such as drought, high salinity and low temperature being also proved as a key phytohormone for the acquisition of postharvest cold tolerance in zucchini fruit. Therefore, it would be of great interest to unravel the mechanisms implicated in the ABA response, using a metabolomic approach. The aim of this work has been to use a combination of metabolomic tools to identify the main metabolic pathways involved in ABA-mediated regulation of chilling tolerance in zucchini fruit. As a result of this study, it was found that ABA modulates the primary metabolism inducing the accumulation of some sugars, organic acids such as succinic acid and amino acids including histidine, serine, phenylalanine, glutamic acid and γ-aminobutyric acid, and that are involved in low-temperature tolerance. ABA treatment also activates the t-zeatin and riboflavin biosynthesis during the first days of cold storage which can be important signals in the ABA-mediated cold response to induce tolerance in zucchini fruit. 2023-06-30T09:12:58Z 2023-06-30T09:12:58Z 2023-06-23 journal article Postharvest abscisic acid treatment modulates the primary metabolism and the biosynthesis of t-zeatin and riboflavin in zucchini fruit exposed to chilling stress. 2023. Postharvest Biology and Technology, 204, 112457 https://hdl.handle.net/10481/83002 https://doi.org/10.1016/j.postharvbio.2023.112457 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Elsevier