Molecules involved in the sperm interaction in the human uterine tube: a histochemical and immunohistochemical approach Cajas, David Carriel Araya, Víctor Campos Muñoz, Antonio Jesús Human uterine tube Epitheliummenstrual cycle Immunohistochemistry Cell adhesion molecules In humans, even where millions of spermatozoa are deposited upon ejaculation in the vagina, only a few thousand enter the uterine tube (UT). Sperm transiently adhere to the epithelial cells lining the isthmus reservoir, and this interaction is essential in coordinating the availability of functional spermatozoa for fertilization. The binding of spermatozoa to the UT epithelium (mucosa) occurs due to interactions between cell-adhesion molecules on the cell surfaces of both the sperm and the epithelial cell. However, in humans, there is little information about the molecules involved. The aim of this study was to perform a histological characterization of the UT focused on determining the tissue distribution and deposition of some molecules associated with cell adhesion (F-spondin, galectin-9, osteopontin, integrin αV/β3) and UT’s contractile activity (TNFα-R1, TNFα- R2) in the follicular and luteal phases. Our results showed the presence of galectin-9, F-spondin, osteopontin, integrin αV/β3, TNFα-R1, and TNFα-R2 in the epithelial cells in ampullar and isthmic segments during the menstrual cycle. Our results suggest that these molecules could form part of the sperm-UT interactions. Future studies will shed light on the specific role of each of the identified molecules 2023-06-12T09:56:48Z 2023-06-12T09:56:48Z 2023 journal article Cajas, D., Guajardo, E., Jara-Rosales, S., Nuñez, C., Vargas, R., Carriel, V., ... & Godoy-Guzman, C. (2023). Molecules involved in the sperm interaction in the human uterine tube: a histochemical and immunohistochemical approach. European Journal of Histochemistry: EJH, 67(2).[doi:10.4081/ejh.2023.3513] https://hdl.handle.net/10481/82343 10.4081/ejh.2023.3513 eng http://creativecommons.org/licenses/by-nc/4.0/ open access Atribución-NoComercial 4.0 Internacional National Library of Medicine