Antioxidant Efficacy and “In Vivo” Safety of a Bentonite/Vitamin C Hybrid Hernández Oliva, Dayaris Sánchez Espejo, Rita María Cerezo González, María Pilar Viseras Iborra, César Antonio César Antonio, Viseras Iborra, Bentonite L-ascorbic acid Antioxidant capacity Photoprotection The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/pharmaceutics15041171/s1, S1: LAA/smectites systems: critical revision; S2: Ophthalmic irritability; S3: Acute dermal toxicity. L-ascorbic acid (LAA), commonly known as vitamin C, is an excellent and recognized antioxidant molecule used in pharmaceutical and cosmetic formulations. Several strategies have been developed in order to preserve its chemical stability, connected with its antioxidant power, but there is little research regarding the employment of natural clays as LAA host. A safe bentonite (Bent)—which was verified by in vivo ophthalmic irritability and acute dermal toxicity assays—was used as carrier of LAA. The supramolecular complex between LAA and clay may constitute an excellent alternative, since the molecule integrity does not seem to be affected, at least from the point of view of its antioxidant capacity. The Bent/LAA hybrid was prepared and characterized through ultraviolet (UV) spectroscopy, X-ray diffraction (XRD), infrared (IR) spectroscopy, thermogravimetric analysis (TG/DTG) and zeta potential measurements. Photostability and antioxidant capacity tests were also performed. The LAA incorporation into Bent clay was demonstrated, as well as the drug stability due to the Bent photoprotective effect onto the LAA molecule. Moreover, the antioxidant capacity of the drug in the Bent/LAA composite was confirmed. 2023-06-07T08:01:15Z 2023-06-07T08:01:15Z 2023-04-07 info:eu-repo/semantics/article Hernández, D.; Montalvo, A.; Pérez, I.; Charnay, C.; Sánchez-Espejo, R.; Cerezo, P.; Viseras, C.; Riela, S.; Cinà, G.; Rivera, A. Antioxidant Efficacy and “In Vivo” Safety of a Bentonite/Vitamin C Hybrid. Pharmaceutics 2023, 15, 1171. [https://doi.org/10.3390/pharmaceutics15041171] https://hdl.handle.net/10481/82307 10.3390/pharmaceutics15041171 eng http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Atribución 4.0 Internacional MDPI