Evaluation of the remineralizing capacity of silver diamine fluoride on demineralized dentin under pH-cycling conditions Cifuentes Jiménez, Carolina Cecilia Bolaños Carmona, María Victoria Enrich Essvein, Tattiana González López, Santiago Álvarez Lloret, Pedro Dentin Sodium fluoride Biomineralization Dental caries Silver compounds Silver diamine Crystallization This study was funded by PID2020-116660GB-I00 by the Ministry of Economy and Competitiveness (MINECO) of the Spanish Government and FEDER A-RNM-408-UGR20. PAL acknowledges the European Regional Development Fund (ERDF) – Next Generation/ EU program. Objective: (1) to determine the effects of the silver diamine fluoride (SDF) and sodium fluoride (NaF) in demineralized dentin exposed to an acid challenge by pH-cycling, (2) to evaluate the remineralizing capacity of SDF/NaF products based on the physicochemical and mechanical properties of the treated dentin surfaces. Methodology: In total, 57 human molars were evaluated in different stages of the experimental period: sound dentin - negative control (Stage 1), demineralized dentin - positive control (Stage 2), and dentin treated with SDF/ NaF products + pH-c (Stage 3). Several commercial products were used for the SDF treatment: Saforide, RivaStar, and Cariestop. The mineral composition and crystalline and morphological characteristics of the dentin samples from each experimental stage were evaluated by infrared spectroscopy (ATR-FTIR), X-ray diffraction, and electron microscopy (SEM-EDX) analytical techniques. Moreover, the mechanical response of the samples was analyzed by means of the three-point bending test. Statistics were estimated for ATR-FTIR variables by Wilcoxon test, while the mechanical data analyses were performed using Kruskal-Wallis and Mann Whitney U tests. Results: Regarding the chemical composition, we observed a higher mineral/organic content in the SDF/NaF treated dentin + pH-c groups (Stage 3) than in the positive control groups (Saforide p=0.03; Cariestop p=0.008; RivaStar p=0.013; NaF p=0.04). The XRD results showed that the crystallite size of hydroxyapatite increased in the SDF/NaF treated dentin + pH-c groups (between +63% in RivaStar to +108% in Saforide), regarding the positive control. SEM images showed that after application of the SDF/NaF products a crystalline precipitate formed on the dentin surface and partially filled the dentin tubules. The flexural strength (MPa) values were higher in the dentin treated with SDF/NaF + pH-c (Stage 3) compared to the positive control groups (Saforide p=0.002; Cariestop p=0.04; RivaStar p=0.04; NaF p=0.02). Conclusions: The application of SDF/ NaF affected the physicochemical and mechanical properties of demineralized dentin. According to the results, the use of SFD/NaF had a remineralizing effect on the dentin surface even under acid challenge. 2023-06-02T07:47:19Z 2023-06-02T07:47:19Z 2023 journal article Cifuentes-Jiménez, C.C. et al. Evaluation of the remineralizing capacity of silver diamine fluoride on demineralized dentin under pH-cycling conditions. J Appl Oral Sci. 2023;31:e20220306. [http://dx.doi.org/10.1590/1678-7757-2022-0306] https://hdl.handle.net/10481/82150 10.1590/1678-7757-2022-0306 eng http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional Universidad Sao Paulo Facultdad de Odontología Bauru