Effects of bisphenol F, bisphenol S, and bisphenol AF on cultured human osteoblasts García Recio, Enrique Costela Ruiz, Víctor Javier Melguizo Rodríguez, Lucía Raquel Ramos Torrecillas, Javier Illescas Montes, Rebeca Luna Bertos, María Elvira De Ruiz Rodríguez, Concepción Bisphenol-F Bisphenol-S Bisphenol AF Osteoblast Cellular viability Cellular differentiation Bisphenol A (BPA) analogs, like BPA, could have adverse effects on human health including bone health. The aim was to determine the effect of BPF, BPS and BPAF on the growth and differentiation of cultured human osteoblasts. Osteoblasts primary culture from bone chips harvested during routine dental work and treated with BPF, BPS, or BPAF for 24 h at doses of 10 –5 , 10 –6 , and 10 –7 M. Next, cell proliferation was studied, apoptosis induction, and alkaline phosphatase (ALP) activity. In addition, mineralization was evaluated at 7, 14, and 21 days of cell culture in an osteogenic medium supplemented with BP analog at the studied doses. BPS treatment inhibited proliferation in a dose-dependent manner at all three doses by inducing apoptosis; BPF exerted a significant inhibitory effect on cell proliferation at the highest dose alone by an increase of apopto- sis; while BPAF had no effect on proliferation or cell viability. Cell differentiation was adversely affected by treatment with BPA analogs in a dose-dependent, observing a reduction in calcium nodule formation at 21 days. According to the results obtained, these BPA analogs could potentially pose a threat to bone health, depending on their concentration in the organism. 2023-05-24T06:37:19Z 2023-05-24T06:37:19Z 2023-05-17 journal article García-Recio, E., Costela-Ruiz, V.J., Melguizo-Rodríguez, L. et al. Effects of bisphenol F, bisphenol S, and bisphenol AF on cultured human osteoblasts. Arch Toxicol (2023). https://hdl.handle.net/10481/81769 https://doi.org/10.1007/s00204-023-03523-2 eng http://creativecommons.org/licenses/by-nc-nd/3.0/ open access Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License Springer Nature