Fibrin and Marine-Derived Agaroses for the Generation of Human Bioartificial Tissues: An Ex Vivo and In Vivo Study Ortiz Arrabal, Olimpia Irastorza Lorenzo, Ainhoa Campos Sánchez, Fernando Martín Piedra, Miguel Ángel Carriel Araya, Víctor Garzón Bello, Ingrid Johanna Ávila-Fernández, Paula Campos Muñoz, Antonio Jesús Chato Astrain, Jesús Alaminos Mingorance, Miguel Tissue engineering Biomaterials Fibrin Agarose Biocompatibility Development of an ideal biomaterial for clinical use is one of the main objectives of current research in tissue engineering. Marine-origin polysaccharides, in particular agaroses, have been widely explored as scaffolds for tissue engineering. We previously developed a biomaterial based on a combination of agarose with fibrin, that was successfully translated to clinical practice. However, in search of novel biomaterials with improved physical and biological properties, we have now generated new fibrin-agarose (FA) biomaterials using 5 different types of agaroses at 4 different concentrations. First, we evaluated the cytotoxic effects and the biomechanical properties of these biomaterials. Then, each bioartificial tissue was grafted in vivo and histological, histochemical and immunohistochemical analyses were performed after 30 days. Ex vivo evaluation showed high biocompatibility and differences in their biomechanical properties. In vivo, FA tissues were biocompatible at the systemic and local levels, and histological analyses showed that biointegration was associated to a pro-regenerative process with M2-type CD206-positive macrophages. These results confirm the biocompatibility of FA biomaterials and support their clinical use for the generation of human tissues by tissue engineering, with the possibility of selecting specific agarose types and concentrations for applications requiring precise biomechanical properties and in vivo reabsorption times. 2023-05-23T11:03:22Z 2023-05-23T11:03:22Z 2023-03-17 info:eu-repo/semantics/article Lorenzo, A.; Campos, F.; Martín-Piedra, M.Á.; Carriel, V.; Garzón, I.; Ávila-Fernández, P.; Frutos, M.J.d.; Esteban, E.; Fernández, J.; et al. Fibrin and Marine-Derived Agaroses for the Generation of Human Bioartificial Tissues: An Ex Vivo and In Vivo Study. Mar. Drugs 2023, 21, 187. [https://doi.org/10.3390/md21030187] https://hdl.handle.net/10481/81763 10.3390/md21030187 eng http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Atribución 4.0 Internacional MDPI