Eggshell Membrane as a Biomaterial for Bone Regeneration Torres Mansilla, Adriana Voltes Martínez, Ana López Ruiz, Elena Marchal Corrales, Juan Antonio Biopolymer Bone biomaterial Bone scaffold Bone tissue engineering Eggshell membrane Organic matrix The physicochemical features of the avian eggshell membrane play an essential role in the process of calcium carbonate deposition during shell mineralization, giving rise to a porous mineralized tissue with remarkable mechanical properties and biological functions. The membrane could be useful by itself or as a bi-dimensional scaffold to build future bone-regenerative materials. This review focuses on the biological, physical, and mechanical properties of the eggshell membrane that could be useful for that purpose. Due to its low cost and wide availability as a waste byproduct of the egg processing industry, repurposing the eggshell membrane for bone bio-material manufacturing fulfills the principles of a circular economy. In addition, eggshell membrane particles have has the potential to be used as bio-ink for 3D printing of tailored implantable scaffolds. Herein, a literature review was conducted to ascertain the degree to which the properties of the eggshell membrane satisfy the requirements for the development of bone scaffolds. In principle, it is biocompatible and non-cytotoxic, and induces proliferation and differentiation of different cell types. Moreover, when implanted in animal models, it elicits a mild inflammatory response and displays characteristics of stability and biodegradability. Furthermore, the eggshell membrane possesses a mechanical viscoelastic behavior comparable to other collagen-based systems. Overall, the biological, physical, and mechanical features of the eggshell membrane, which can be further tuned and improved, make this natural polymer suitable as a basic component for developing new bone graft materials 2023-05-18T06:31:26Z 2023-05-18T06:31:26Z 2023-03-08 journal article Torres-Mansilla, A.; Hincke, M.; Voltes, A.; López-Ruiz, E.; Baldión, P.A.; Marchal, J.A.; Álvarez-Lloret, P.; Gómez-Morales, J. Eggshell Membrane as a Biomaterial for Bone Regeneration. Polymers 2023, 15, 1342. [https://doi.org/10.3390/ polym15061342] https://hdl.handle.net/10481/81622 10.3390/ polym15061342 eng info:eu-repo/grantAgreement/EC/Nest Generation EU/AYUD/2022/33525 (FICYT). http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional MDPI