<rdf:RDF xmlns:rdf="http://www.openarchives.org/OAI/2.0/rdf/" xmlns:ow="http://www.ontoweb.org/ontology/1#" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:ds="http://dspace.org/ds/elements/1.1/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:doc="http://www.lyncode.com/xoai" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/rdf/ http://www.openarchives.org/OAI/2.0/rdf.xsd">
   <ow:Publication rdf:about="oai:digibug.ugr.es:10481/80883">
      <dc:title>Tandem Repeat DNA Provides Many Cytological Markers for Hybrid Zone Analysis in Two Subspecies of the Grasshopper Chorthippus parallelus</dc:title>
      <dc:creator>Navarro Domínguez, Beatriz María</dc:creator>
      <dc:creator>Cabrero Hurtado, Josefa</dc:creator>
      <dc:creator>López León, María Dolores</dc:creator>
      <dc:creator>Martínez Camacho, Juan Pedro</dc:creator>
      <dc:subject>Chorthippus parallelus</dc:subject>
      <dc:subject>Hybrid zone</dc:subject>
      <dc:subject>Repetitive DNA</dc:subject>
      <dc:subject>Tandem repeats</dc:subject>
      <dc:subject>FISH</dc:subject>
      <dc:description>Recent advances in next generation sequencing (NGS) have greatly increased our understanding&#xd;
of non-coding tandem repeat (TR) DNA. Here we show how TR DNA can be useful for&#xd;
the study of hybrid zones (HZ), as it serves as a marker to identify introgression in areas where&#xd;
two biological entities come in contact. We used Illumina libraries to analyse two subspecies of&#xd;
the grasshopper Chorthippus parallelus, which currently form a HZ in the Pyrenees. We retrieved a&#xd;
total of 152 TR sequences, and used fluorescent in situ hybridization (FISH) to map 77 families in&#xd;
purebred individuals from both subspecies. Our analysis revealed 50 TR families that could serve&#xd;
as markers for analysis of this HZ, using FISH. Differential TR bands were unevenly distributed&#xd;
between chromosomes and subspecies. Some of these TR families yielded FISH bands in only one of&#xd;
the subspecies, suggesting the amplification of these TR families after the geographic separation of&#xd;
the subspecies in the Pleistocene. Our cytological analysis of two TR markers along a transect of the&#xd;
Pyrenean hybrid zone showed asymmetrical introgression of one subspecies into the other, consistent&#xd;
with previous findings using other markers. These results demonstrate the reliability of TR-band&#xd;
markers for hybrid zone studies.</dc:description>
      <dc:date>2023-03-28T06:46:16Z</dc:date>
      <dc:date>2023-03-28T06:46:16Z</dc:date>
      <dc:date>2023-02-03</dc:date>
      <dc:type>journal article</dc:type>
      <dc:identifier>Navarro-Domínguez, B... [et al.]. Tandem Repeat DNA Provides Many Cytological Markers for Hybrid Zone Analysis in Two Subspecies of the Grasshopper Chorthippus parallelus. Genes 2023, 14, 397. [https://doi.org/10.3390/genes14020397]</dc:identifier>
      <dc:identifier>https://hdl.handle.net/10481/80883</dc:identifier>
      <dc:identifier>10.3390/genes14020397</dc:identifier>
      <dc:language>eng</dc:language>
      <dc:relation>info:eu-repo/grantAgreement/EC/H2020/875732</dc:relation>
      <dc:rights>http://creativecommons.org/licenses/by/4.0/</dc:rights>
      <dc:rights>open access</dc:rights>
      <dc:rights>Atribución 4.0 Internacional</dc:rights>
      <dc:publisher>MDPI</dc:publisher>
   </ow:Publication>
</rdf:RDF>