Expression of Basement Membrane Molecules by Wharton Jelly Stem Cells (WJSC) in Full-Term Human Umbilical Cords, Cell Cultures and Microtissues Sánchez Porras, David Durand Herrera, Daniel Carmona Martos, Ramón Blanco Elices, Cristina Garzón Bello, Ingrid Johanna Alaminos Mingorance, Miguel García García, Óscar Darío Chato Astrain, Jesús Carriel Araya, Víctor Umbilical cord (UC) Wharton jelly stem cells (WJSC) Extracellular matrix (ECM) Basement membrane (BM) Basal lamina (BL) stem cell-based microtissues (MT) Tissue engineering (TE) Wharton’s jelly stem cells (WJSC) from the human umbilical cord (UC) are one of the most promising mesenchymal stem cells (MSC) in tissue engineering (TE) and advanced therapies. The cell niche is a key element for both, MSC and fully differentiated tissues, to preserve their unique features. The basement membrane (BM) is an essential structure during embryonic development and in adult tissues. Epithelial BMs are well-known, but similar structures are present in other histological structures, such as in peripheral nerve fibers, myocytes or chondrocytes. Previous studies suggest the expression of some BM molecules within the Wharton’s Jelly (WJ) of UC, but the distribution pattern and full expression profile of these molecules have not been yet elucidated. In this sense, the aim of this histological study was to evaluate the expression of main BM molecules within the WJ, cultured WJSC and during WJSC microtissue (WJSC-MT) formation process. Results confirmed the presence of a pericellular matrix composed by the main BM molecules—collagens (IV, VII), HSPG2, agrin, laminin and nidogen—around the WJSC within UC. Additionally, ex vivo studies demonstrated the synthesis of these BM molecules, except agrin, especially during WJSC-MT formation process. The WJSC capability to synthesize main BM molecules could offer new alternatives for the generation of biomimetic-engineered substitutes where these molecules are particularly needed. 2023-03-27T10:03:52Z 2023-03-27T10:03:52Z 2023-02-15 journal article Sánchez-Porras, D... [et al.]. Expression of Basement Membrane Molecules by Wharton Jelly Stem Cells (WJSC) in Full-Term Human Umbilical Cords, Cell Cultures and Microtissues. Cells 2023, 12, 629. [https://doi.org/10.3390/cells12040629] https://hdl.handle.net/10481/80864 10.3390/cells12040629 eng http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional MDPI