Polyethylenimine-Bisphosphonate-Cyclodextrin Ternary Conjugates: Supramolecular Systems for the Delivery of Antineoplastic Drugs Plesselova, Simona García Cerezo, Pablo Blanco Suárez, Víctor Reche Pérez, Francisco José Hernández Mateo, Fernando Santoyo González, Francisco Girón González, María Dolores Salto González, Rafael Bisphosphonates (BPs) are bone-binding molecules that provide targeting capabilities to bone cancer cells when conjugated with drug-carrying polymers. This work reports the design, synthesis, and biological evaluation of polyethyleneimine− BP−cyclodextrin (PEI-BP-CD) ternary conjugates with supramolecular capabilities for the loading of antineoplastic drugs. A straightforward, modular, and versatile strategy based on the click aza-Michael addition reaction of vinyl sulfones (VSs) allows the grafting of BPs targeting ligands and βCD carrier appendages to the PEI polymeric scaffold. The in vitro evaluation (cytotoxicity, cellular uptake, internalization routes, and subcellular distribution) for the ternary conjugates and their doxorubicin inclusion complexes in different bone-related cancer cell lines (MC3T3-E1 osteoblasts, MG-63 sarcoma cells, and MDA-MB-231 breast cancer cells) confirmed specificity, mitochondrial targeting, and overall capability to mediate a targeted drug transport to those cells. The in vivo evaluation using xenografts of MG-63 and MDA-MB-231 cells on mice also confirmed the targeting of the conjugates. 2023-03-08T10:10:46Z 2023-03-08T10:10:46Z 2021-08-09 journal article J. Med. Chem. 2021, 64, 12245−12260. [https://doi.org/10.1021/acs.jmedchem.1c00887] https://hdl.handle.net/10481/80467 10.1021/acs.jmedchem.1c00887 eng http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional American Chemical Society