Plasma Levels of Omega-3 and Omega-6 Derived Oxylipins Are Associated with Fecal Microbiota Composition in Young Adults Xu, Huiwen Jurado Fasoli, Lucas Ortiz Álvarez, Lourdes Osuna Prieto, Francisco Javier Plaza Díaz, Julio Gil Hernández, Ángel Ruiz Ruiz, Jonatan Martínez Téllez, Borja Manuel Gut microbiota Inflammation Intestinal alkaline phosphatase Microbiome PUFAs Pre-clinical studies suggest that circulating oxylipins, i.e., the oxidation products of polyunsaturated fatty acids (PUFAs), modulate gut microbiota composition in mice, but there is no information available in humans. Therefore, this study aimed to investigate the relationship between omega-3 and omega-6 derived oxylipins plasma levels and fecal microbiota composition in a cohort of young adults. 80 young adults (74% women; 21.9 +/- 2.2 years old) were included in this cross-sectional study. Plasma levels of oxylipins were measured using liquid chromatography-tandem mass spectrometry. Fecal microbiota composition was analyzed by V3-V4 16S rRNA gene sequencing. We observed that plasma levels of omega-3 derived oxylipins were positively associated with the relative abundance of Clostridium cluster IV genus (Firmicutes phylum; rho >= 0.415, p <= 0.009) and negatively associated with the relative abundance of Sutterella genus (Proteobacteria phylum; rho >= -0.270, p <= 0.041), respectively. Moreover, plasma levels of omega-6 derived oxylipins were negatively associated with the relative abundance of Acidaminococcus and Phascolarctobacterium genera (Firmicutes phylum; all rho >= -0.263, p <= 0.024), as well as Sutterella, Succinivibrio, and Gemmiger genera (Proteobacteria phylum; all rho >= -0.263, p <= 0.024). Lastly, the ratio between omega-6 and omega-3 oxylipins plasma levels was negatively associated with the relative abundance of Clostridium cluster IV genus (Firmicutes phylum; rho = -0.334, p = 0.004) and Butyricimonas genus (Bacteroidetes phylum; rho = -0.292, p = 0.014). In conclusion, our results show that the plasma levels of omega-3 and omega-6 derived oxylipins are associated with the relative abundance of specific fecal bacteria genera. 2023-01-17T13:32:05Z 2023-01-17T13:32:05Z 2022-11-24 journal article Xu, H... [et al.]. Plasma Levels of Omega-3 and Omega-6 Derived Oxylipins Are Associated with Fecal Microbiota Composition in Young Adults. Nutrients 2022, 14, 4991. [https://doi.org/10.3390/nu14234991] https://hdl.handle.net/10481/79065 10.3390/nu14234991 eng http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional MDPI