<rdf:RDF xmlns:rdf="http://www.openarchives.org/OAI/2.0/rdf/" xmlns:ow="http://www.ontoweb.org/ontology/1#" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:ds="http://dspace.org/ds/elements/1.1/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:doc="http://www.lyncode.com/xoai" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/rdf/ http://www.openarchives.org/OAI/2.0/rdf.xsd">
   <ow:Publication rdf:about="oai:digibug.ugr.es:10481/78089">
      <dc:title>Trypanosoma cruzi pathogenicity involves virulence factor expression and upregulation of bioenergetic and biosynthetic pathways</dc:title>
      <dc:creator>San Francisco, Juan</dc:creator>
      <dc:creator>Osuna Carrillo De Albornoz, Antonio</dc:creator>
      <dc:creator>Vílchez Tornero, Susana</dc:creator>
      <dc:subject>Trypanosoma cruzi</dc:subject>
      <dc:subject>Virulence</dc:subject>
      <dc:subject>Genetically related cell lines</dc:subject>
      <dc:subject>Proteomics</dc:subject>
      <dc:description>The molecular repertoire of Trypanosoma cruzi effects its virulence and impacts the clinical course&#xd;
of the resulting Chagas disease. This study aimed to determine the mechanism underlying the&#xd;
pathogenicity of T. cruzi. Two T. cruzi cell lines (C8C3hvir and C8C3lvir), obtained from the clone&#xd;
H510 C8C3 and exhibiting different virulence phenotypes, were used to evaluate the parasite’s&#xd;
infectivity in mice. The organ parasite load was analysed by qPCR. The proteomes of both T. cruzi&#xd;
cell lines were compared using nLC-MS/MS. Cruzipain (Czp), complement regulatory protein (CRP),&#xd;
trans-sialidase (TS), Tc-85, and sialylated epitope expression levels were evaluated by immunoblotting.&#xd;
High-virulence C8C3hvir was highly infectious in mice and demonstrated three to five&#xd;
times higher infectivity in mouse myocardial cells than low-virulence C8C3lvir. qPCR revealed&#xd;
higher parasite loads in organs of acute as well as chronically C8C3hvir-infected mice than in&#xd;
those of C8C3lvir-infected mice. Comparative quantitative proteomics revealed that 390 of 1547&#xd;
identified proteins were differentially regulated in C8C3hvir with respect to C8C3lvir. Amongst&#xd;
these, 174 proteins were upregulated in C8C3hvir and 216 were downregulated in C8C3lvir. The&#xd;
upregulated proteins in C8C3hvir were associated with the tricarboxylic acid cycle, ribosomal&#xd;
proteins, and redoxins. Higher levels of Czp, CRP, TS, Tc-85, and sialylated epitopes were&#xd;
expressed in C8C3hvir than in C8C3lvir. Thus, T. cruzi virulence may be related to virulence factor&#xd;
expression as well as upregulation of bioenergetic and biosynthetic pathways proteins.</dc:description>
      <dc:date>2022-11-23T09:54:13Z</dc:date>
      <dc:date>2022-11-23T09:54:13Z</dc:date>
      <dc:date>2022-10-25</dc:date>
      <dc:type>journal article</dc:type>
      <dc:identifier>Juan San Francisco... [et al.] (2022) Trypanosoma cruzi pathogenicity involves virulence factor expression and upregulation of bioenergetic and biosynthetic pathways, Virulence, 13:1, 1827-1848, DOI: [10.1080/21505594.2022.2132776]</dc:identifier>
      <dc:identifier>https://hdl.handle.net/10481/78089</dc:identifier>
      <dc:identifier>10.1080/21505594.2022.2132776</dc:identifier>
      <dc:language>eng</dc:language>
      <dc:rights>http://creativecommons.org/licenses/by/4.0/</dc:rights>
      <dc:rights>open access</dc:rights>
      <dc:rights>Atribución 4.0 Internacional</dc:rights>
      <dc:publisher>Taylor &amp; Francis</dc:publisher>
   </ow:Publication>
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