Fecal microbiota composition is related to brown adipose tissue 18F‑fluorodeoxyglucose uptake in young adults Ortiz Álvarez, Lourdes Acosta, F. M. Xu, Huiwen Sánchez Delgado, Guillermo Plaza Díaz, Julio Llamas Elvira, José Manuel Gil Hernández, Ángel Ruiz Ruiz, Jonatan Martínez Téllez, Borja Manuel Brown fat Glucose uptake Gut microbiota Obesity Short-chain fatty acids Objective Human brown adipose tissue (BAT) has gained considerable attention as a potential therapeutic target for obesity and its related cardiometabolic diseases; however, whether the gut microbiota might be an efficient stimulus to activate BAT metabolism remains to be ascertained. We aimed to investigate the association of fecal microbiota composition with BAT volume and activity and mean radiodensity in young adults. Methods 82 young adults (58 women, 21.8 ± 2.2 years old) participated in this cross-sectional study. DNA was extracted from fecal samples and 16S rRNA sequencing was performed to analyse the fecal microbiota composition. BAT was determined via a static 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography-computed tomography scan (PET/CT) after a 2 h personalized cooling protocol. 18F-FDG uptake was also quantified in white adipose tissue (WAT) and skeletal muscles. Results The relative abundance of Akkermansia, Lachnospiraceae sp. and Ruminococcus genera was negatively correlated with BAT volume, BAT SUVmean and BAT SUVpeak (all rho ≤ − 0.232, P ≤ 0.027), whereas the relative abundance of Bifidobacterium genus was positively correlated with BAT SUVmean and BAT SUVpeak (all rho ≥ 0.262, P ≤ 0.012). On the other hand, the relative abundance of Sutterellaceae and Bifidobacteriaceae families was positively correlated with 18FFDG uptake by WAT and skeletal muscles (all rho ≥ 0.213, P ≤ 0.042). All the analyses were adjusted for the PET/CT scan date as a proxy of seasonality. Conclusion Our results suggest that fecal microbiota composition is involved in the regulation of BAT and glucose uptake by other tissues in young adults. Further studies are needed to confirm these findings. 2022-11-04T12:30:15Z 2022-11-04T12:30:15Z 2022-10-15 journal article Ortiz-Alvarez, L... [et al.]. Fecal microbiota composition is related to brown adipose tissue 18F-fluorodeoxyglucose uptake in young adults. J Endocrinol Invest (2022). [https://doi.org/10.1007/s40618-022-01936-x] https://hdl.handle.net/10481/77755 10.1007/s40618-022-01936-x eng http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional Springer