Different Effects of Low Selenite and Selenium-Nanoparticle Supplementation on Adipose Tissue Function and Insulin Secretion in Adolescent Male Rats Ojeda, María Luisa Moreno Fernández, Jorge Díaz Castro, Javier Selenite Nanoparticles Adipose tissue Insulin Adolescence is a period of intense growth and endocrine changes, and obesity and insulinresistance processes during this period have lately been rising. Selenium (Se) homeostasis is related to lipid metabolism depending on the form and dose of Se. This study tests the actions of low-dose selenite and Se nanoparticles (SeNPs) on white (WAT) and brown adipose tissue (BAT) deposition, insulin secretion, and GPx1, IRS-1 and FOXO3a expression in the WAT of adolescent rats as regards oxidative stress, adipocyte length and adipokine secretion. Four groups of male adolescent rats were treated: control (C), low selenite supplementation (S), low SeNP supplementation (NS) and moderate SeNP supplementation (NSS). Supplementation was received orally through water intake; NS and NSS rats received two- and tenfold more Se than C animals, respectively. SeNPs were obtained by reducing Se tetrachloride in the presence of ascorbic acid. For the first time in vivo, it was demonstrated that low selenite supplementation contributed to increased adipogenesis via the insulin signaling pathway and LCN2 modulation, while low SeNP administration prevented fat depots inWAT via the decrease in insulin signaling and FOXO3a autophagy inWAT, lowering inflammation. These effects were independent of GPx1 expression or activity in WAT. These findings provide data for dietary approaches to prevent obesity and/or anorexia during adolescence. These findings may be relevant to future studies looking at a nutritional approach aimed at pre-venting obesity and/or anorexia in adolescence. 2022-09-30T07:10:20Z 2022-09-30T07:10:20Z 2022-08-30 journal article Ojeda,M.L... [et al.]. Different Effects of Low Selenite and Selenium- Nanoparticle Supplementation on Adipose Tissue Function and Insulin Secretion in Adolescent Male Rats. Nutrients 2022, 14, 3571. [https://doi.org/10.3390/nu14173571] https://hdl.handle.net/10481/77088 10.3390/nu14173571 eng http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional Atribución 4.0 Internacional MDPI