Optical Behavior of Human Skin Substitutes: Absorbance in the 200–400 nm UV Range Ruiz López, Javier Cardona Pérez, Juan De La Cruz Garzón Bello, Ingrid Johanna Pérez Gómez, María Del Mar Alaminos Mingorance, Miguel Chato Astrain, Jesús Ionescu, Ana María Andreea Absorption UV radiation Bioengineered skin Fibrin-agarose biomaterial The most recent generation of bioengineered human skin allows for the efficient treatment of patients with severe skin defects. Despite UV sunlight can seriously affect human skin, the optical behavior in the UV range of skin models is still unexplored. In the present study, absorbance and transmittance of the UGRSKIN bioartificial skin substitute generated with human skin cells combined with fibrin-agarose biomaterials were evaluated for: UV-C (200–280 nm), -B (280–315 nm), and -A (315–400 nm) spectral range after 7, 14, 21 and 28 days of ex vivo development. The epidermis of the bioartificial skin substitute was able to mature and differentiate in a time-dependent manner, expressing relevant molecules able to absorb most of the incoming UV radiation. Absorbance spectral behavior of the skin substitutes showed similar patterns to control native skin (VAF > 99.4%), with values 0.85–0.90 times lower than control values at 7 and 14- days and 1.05–1.10 times the control values at 21- and 28-days. UV absorbance increased, and UV transmission decreased with culture time, and comparable results to the control were found at 21 and 28 days. These findings support the use of samples corresponding to 21 or 28 days of development for clinical purposes due to their higher histological similarities with native skin, but also because of their absorbance of UV radiation. 2022-09-12T07:42:12Z 2022-09-12T07:42:12Z 2022-07-08 info:eu-repo/semantics/article Ruiz-López, J... [et al.]. Optical Behavior of Human Skin Substitutes: Absorbance in the 200–400 nm UV Range. Biomedicines 2022, 10, 1640. [https://doi.org/10.3390/biomedicines10071640] http://hdl.handle.net/10481/76621 10.3390/biomedicines10071640 eng http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Atribución 4.0 Internacional MDPI