Maltodextrin-amino acids electrospun scaffolds cross-linked with Maillard-type reaction for skin tissue engineering Ruggeri, Marco Sánchez Espejo, Rita María Viseras Iborra, César Antonio Chronic wounds Maltodextrin Amino acids Maillard-type reaction Mechanical properties Surface zeta potential Immune response Murine bum/excisional model The goal of this work is the design and the development of scaffolds based on maltodextrin (MD) to recover chronic lesions. MD was mixed with arginine/lysine/polylysine and the electrospinning was successfully used to prepare scaffolds with uniform and continuous nanofibers having regular shape and smooth surface. A thermal treatment was applied to obtain insoluble scaffolds in aqueous environment, taking the advantage of amino acids-polysaccharide conjugates formed via Maillard-type reaction. The morphological analysis showed that the scaffolds had nanofibrous structures, and that the cross-linking by heating did not significantly change the nanofibers' dimensions and did not alter the systemstability. Moreover, the heating process caused a reduction of free amino group and proportionally increased scaffold cross-linking degree. The scaffolds were elastic and resistant to break, and possessed negative zeta potential in physiological fluids. These were characterized by direct antioxidant properties and Fe2+ chelation capability (indirect antioxidant properties). Moreover, the scaffolds were cytocompatible towards fibroblasts and monocytesderived macrophages, and did not show any significant pro-inflammatory activity. Finally, those proved to accelerate the recovery of the burn/excisional wounds. Considering all the features, MD-poly/amino acids scaffolds could be considered as promising medical devices for the treatment of chronic wounds. 2022-07-08T08:46:08Z 2022-07-08T08:46:08Z 2021-12-03 journal article Marco Ruggeri... [et al.]. Maltodextrin-amino acids electrospun scaffolds cross-linked with Maillard-type reaction for skin tissue engineering, Biomaterials Advances, Volume 133, 2022, 112593, ISSN 2772-9508, [https://doi.org/10.1016/j.msec.2021.112593] http://hdl.handle.net/10481/75884 10.1016/j.msec.2021.112593 eng info:eu-repo/grantAgreement/EC/H2020/814607 http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional Elsevier