Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98-KDM5A Fusion Gene in the AAVS1 Safe Harbor Locus Domingo Reinés, Joan Martínez Navajas, Gonzalo Montes, Rosa Lamolda, Mar Simón Sáez, Iris Ríos Pelegrina, Rosa María López Hidalgo, Javier Luis García Del Moral Garrido, Raimundo Marchal Corrales, Juan Antonio Real Luna, Pedro José Ramos Mejía, Verónica Acute myeliod leukemia Pediatric Disease model Human stem cell Hematopoieisis Pediatric acute myeloid leukemia (AML) is a rare and heterogeneous disease that remains the major cause of mortality in children with leukemia. To improve the outcome of pediatric AML we need to gain knowledge on the biological bases of this disease. NUP98-KDM5A (NK5A) fusion protein is present in a particular subgroup of young pediatric patients with poor outcome. We report the generation and characterization of human Embryonic Stem Cell (hESC) clonal lines with inducible expression of NK5A. Temporal control of NK5A expression during hematopoietic differentiation from hESC will be critical for elucidating its participation during the leukemogenic process. 2022-07-06T10:28:23Z 2022-07-06T10:28:23Z 2022-06-01 journal article Domingo-Reinés J... [et al.] (2022) Generation of a H9 Clonal Cell Line With Inducible Expression of NUP98- KDM5A Fusion Gene in the AAVS1 Safe Harbor Locus. Front. Cell Dev. Biol. 10:846092. doi: [10.3389/fcell.2022.846092] http://hdl.handle.net/10481/75851 10.3389/fcell.2022.846092 eng http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional Frontiers