An oleuropein rich-olive (Olea europaea L.) leaf extract reduces β-amyloid and tau proteotoxicity through regulation of oxidative- and heat shock-stress responses in Caenorhabditis elegans Romero Márquez, José Manuel Navarro Hortal, María Dolores Jiménez Trigo, Victoria Vera Ramírez, Laura Forbes Hernández, Tamara Yuliett Esteban Muñoz, Adelaida Sánchez González, Cristina Quiles Morales, José Luis Alzheimer disease DAF-16/FOXO HSP-16.2 IIS pathway RNAi SKN-1/NRF2 Olive tree-derived products have been associated with numerous benefits for health. The aim of the present study was to characterize an olive leaf extract enriched in oleuropein (OLE) concerning phenolic content and profile as well as antioxidant capacity. Short-term and long-term toxicity, including oxidative stress, was in vivo evaluated in the experimental model Caenorhabditis elegans. Moreover, the potential therapeutic effect of the extract against Aβ induced- and tau protein induced-toxicity was also evaluated in C. elegans. OLE treatment did not exert toxicity. On the contrary, the extract was able to ameliorate oxidative stress and proteotoxicity related to Aβ and tau aggregation. The potential molecular mechanisms present behind the observed results explored by RNAi technology revealed that DAF-16/FOXO and SKN-1/NRF2, elements of the insulin insulin-like signalling pathway, as well as HSP-16.2 overexpression were involved. 2022-06-28T07:26:46Z 2022-06-28T07:26:46Z 2022-03-08 journal article Jose M. Romero-Márquez... [et al.]. An oleuropein rich-olive (Olea europaea L.) leaf extract reduces β-amyloid and tau proteotoxicity through regulation of oxidative- and heat shock-stress responses in Caenorhabditis elegans, Food and Chemical Toxicology, Volume 162, 2022, 112914, ISSN 0278-6915, [https://doi.org/10.1016/j.fct.2022.112914] http://hdl.handle.net/10481/75687 10.1016/j.fct.2022.112914 eng http://creativecommons.org/licenses/by/3.0/es/ open access Atribución 3.0 España Elsevier