Effects of sulphur amino acids on the size and structure of microbial communities of aerobic granular sludge bioreactors Rosa Masegosa, Aurora Pérez Bou, Lizandra Muñoz Palazón, Barbara Monteoliva Garcia, Antonio González Martínez, Alejandro González López, Jesús Juan Correa Galeote, David Cysteine Methionine qPCR Illumina sequencing N-removal AGS-SBR Granular activated sludge has been described as a promising tool in treating wastewater. However, the effect of high concentrations of sulphur amino acids, cysteine and methionine, in the evolution, development and stability of AGS-SBRs (aerobic granular sludge in sequential batch reactors) and their microbial communities is not well-established. Therefore, this study aimed to evaluate microbial communities' size, structure and dynamics in two AGS-SBRs fed with two different concentrations of amino acids (50 and 100 mg L− 1 of both amino acids). In addition, the impact of the higher level of amino acids was also determined under an acclimatization or shock strategy. While N removal efficiency decreased with amino acids, the removal of the organic matter was generally satisfactory. Moreover, the abrupt presence of both amino acids reduced even further the removal performance of N, whereas under progressive adaptation, the removal yield was higher. Besides, excellent removal rates of cysteine and methionine elimination were found, in all stages below 80% of the influent values. Generally considered, the addition of amino acids weakly impacts the microbial communities' total abundances. On the contrary, the presence of amino acids sharply modulated the dominant bacterial structures. Furthermore, the highest amino acid concentration under the shock strategy resulted in a severe change in the structure of the microbial community. Acidovorax, Flavobacterium, Methylophilus, Stenotrophomonas and Thauera stood out as the prominent bacteria to cope with the high presence of cysteine and methionine. Hence, the AGS-SBR technology is valuable for treating influents enriched in sulphur Aa inclusively when a shock strategy was used. 2022-06-27T10:26:27Z 2022-06-27T10:26:27Z 2022-05-25 journal article Rosa-Masegosa, A... [et al.]. Effects of sulphur amino acids on the size and structure of microbial communities of aerobic granular sludge bioreactors. Amino Acids (2022). [https://doi.org/10.1007/s00726-022-03168-y] http://hdl.handle.net/10481/75678 10.1007/s00726-022-03168-y eng http://creativecommons.org/licenses/by/3.0/es/ open access Atribución 3.0 España Springer