<rdf:RDF xmlns:rdf="http://www.openarchives.org/OAI/2.0/rdf/" xmlns:ow="http://www.ontoweb.org/ontology/1#" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:ds="http://dspace.org/ds/elements/1.1/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:doc="http://www.lyncode.com/xoai" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/rdf/ http://www.openarchives.org/OAI/2.0/rdf.xsd">
   <ow:Publication rdf:about="oai:digibug.ugr.es:10481/75672">
      <dc:title>Anti-Cancerous Potential of Polysaccharides Derived from Wheat Cell Culture</dc:title>
      <dc:creator>Murtazina, Alima</dc:creator>
      <dc:creator>Ruiz Alcalá, Gloria</dc:creator>
      <dc:creator>Jiménez Martínez, Yaiza</dc:creator>
      <dc:creator>Marchal Corrales, Juan Antonio</dc:creator>
      <dc:creator>Boulaiz Tassi, Houria</dc:creator>
      <dc:subject>Wheat cell culture</dc:subject>
      <dc:subject>Plant polysaccharides</dc:subject>
      <dc:subject>Colon cancer cells</dc:subject>
      <dc:subject>Inhibition of proliferation</dc:subject>
      <dc:subject>Electron microscopy</dc:subject>
      <dc:subject>Apoptosis</dc:subject>
      <dc:subject>Cytochrome c</dc:subject>
      <dc:subject>Differentiation</dc:subject>
      <dc:description>There is a global need to discover effective anti-cancerous compounds from natural sources.&#xd;
Cultivated wheat cells can be a valuable source of non-toxic or low toxic plant-derived polysaccharides.&#xd;
In this study, we evaluated the anti-cancer ability of seven fractions of wheat cell culture&#xd;
polysaccharides (WCCPSs) in the HCT-116 colon cancer cell line. Almost all (6/7) fractions had&#xd;
an inhibitory effect on the proliferation of colon cancer cells, and two fractions (A-b and A-f) had&#xd;
considerable therapeutic indexes. The WCCPS fractions induced cell cycle arrest in the G1 phase&#xd;
and induced different rates of apoptosis ( 48%). Transmission and scanning electron microscopy&#xd;
revealed that WCCPS fractions caused apoptotic changes in the nucleus and cytoplasm, including&#xd;
damage to mitochondria and external morphological signs of apoptosis. In addition, the WCCPSs&#xd;
induced an increase in the levels of Bax, cytochrome c, and caspases 8 and 3, indicating that cell&#xd;
death progressed through intrinsic and extrinsic pathways of apoptosis. Furthermore, some fractions&#xd;
caused a significant decrease of c-Myc, b-catenin, NFkB2, and HCAM (CD 44) levels, indicating&#xd;
enhanced cell differentiation. Thus, for the first time, our results provide a proof of concept of the&#xd;
anti-cancer capacity of WCCPS fractions in colorectal cancer.</dc:description>
      <dc:date>2022-06-27T08:33:24Z</dc:date>
      <dc:date>2022-06-27T08:33:24Z</dc:date>
      <dc:date>2022-05-20</dc:date>
      <dc:type>journal article</dc:type>
      <dc:identifier>Murtazina, A... [et al.]. Anti-Cancerous Potential of Polysaccharides Derived from Wheat Cell Culture. Pharmaceutics 2022, 14, 1100. [https://doi.org/10.3390/pharmaceutics14051100]</dc:identifier>
      <dc:identifier>http://hdl.handle.net/10481/75672</dc:identifier>
      <dc:identifier>10.3390/pharmaceutics14051100</dc:identifier>
      <dc:language>eng</dc:language>
      <dc:rights>http://creativecommons.org/licenses/by/3.0/es/</dc:rights>
      <dc:rights>open access</dc:rights>
      <dc:rights>Atribución 3.0 España</dc:rights>
      <dc:publisher>MDPI</dc:publisher>
   </ow:Publication>
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