In Vivo Nutritional Assessment of the Microalga Nannochloropsis gaditana and Evaluation of the Antioxidant and Antiproliferative Capacity of Its Functional Extracts Martínez Martínez, Rosario García Beltrán, Alejandro Kapravelou, Garyfallia Mesas Hernández, Cristina Cabeza Montilla, Laura Perazzoli, Gloria Galisteo Moya, Milagros Aranda Ramírez, Pilar Prados Salazar, José Carlos López-Jurado Romero De La Cruz, María Melguizo Alonso, Consolación Porres Foulquie, Jesús María Nannochloropsis gaditana Functional extracts Nutrient bioavailability Antioxidant capacity This research was funded by the International Excellence Campus of the Sea (CEI-MAR) through Project CEIJ-016, as well as the Spanish Ministry of Science, Innovation and Universities and the European Union through projects RTC-2017-6540-1 and RTI-2018-100934-B-I00 and the FEDER program, respectively. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. Nannochloropsis gaditana is a microalga with interesting nutritional and functional value due to its high content of protein, polyunsaturated fatty acids, and bioactive compounds. However, the hardness of its cell wall prevents accessibility to these components. This work aimed to study the effect of a treatment to increase the fragility of the cell wall on the bioavailability of its nutrients and functional compounds. The antioxidant and antiproliferative capacity of functional extracts from treated and untreated N. gaditana was assessed, and the profile of bioactive compounds was characterized. Furthermore, to study the effect of treatment on its nutrient availability and functional capacity, an in vivo experiment was carried out using a rat experimental model and a 20% dietary inclusion level of microalgae. Functional extracts from treated N. gaditana exhibited higher antioxidant activity than the untreated control. Furthermore, the treated microalga induced hypoglycemic action, higher nitrogen digestibility, and increased hepatic antioxidant activity. In conclusion, N. gaditana has interesting hepatoprotective, antioxidant, and anti-inflammatory potential, thus proving itself an ideal functional food candidate, especially if the microalga is treated to increase the fragility of its cell wall before consumption. 2022-06-27T08:09:40Z 2022-06-27T08:09:40Z 2022-05-11 info:eu-repo/semantics/article Martínez, R... [et al.]. In Vivo Nutritional Assessment of the Microalga Nannochloropsis gaditana and Evaluation of the Antioxidant and Antiproliferative Capacity of Its Functional Extracts. Mar. Drugs 2022, 20, 318. [https://doi.org/10.3390/md20050318] http://hdl.handle.net/10481/75668 10.3390/md20050318 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España MDPI