Nanoencapsulated Boron Foliar Supply Increased Expression of NIPs Aquaporins and BOR Transporters of In Vitro Ipomoea batatas Plants Nicolás Espinosa, Juan Ríos Ruiz, Juan José Aquaporins Boron Boron transporters Metabolomic analysis Mineral nutrition In vitro culture Ipomoea batatas This work was supported by the Spanish Ministry of Science and Innovation [RTC-2017- 6544-2]. Nanoencapsulation with proteoliposomes from natural membranes has been proposed as a carrier for the highly efficient delivery of mineral nutrients into plant tissues. Since Boron deficiency occurred frequently in crops, and is an element with low movement in tissues, in this work, nanoencapsulated B vs free B was applied to in vitro sweet potato plants to investigate the regulation of B transporters (aquaporins and specific transporters). Additionally, an metabolomic analysis was performed, and mineral nutrient and pigment concentrations were determined. The results showed high increases in B concentration in leaves when B was applied as encapsulated, but also Fe and Mn concentration increased. Likewise, the metabolomics study showed that single carbohydrates of these plants could be related to the energy need for increasing the expression of most NIP aquaporins (NIP1;2, NIP1;3; NIP4;1, NIP4;2, NIP5;1, NIP6;1, and NIP7) and boron transporters (BOR2, BOR4 and BOR7;1). Therefore, the results were associated with the higher mobility of encapsulated B into leaves and the stimulation of transport into cells, since after applying encapsulated B, the aforementioned NIPs and BORs increased in expression. 2022-03-29T12:07:56Z 2022-03-29T12:07:56Z 2022-02-09 info:eu-repo/semantics/article Nicolas-Espinosa, J... [et al.]. Nanoencapsulated Boron Foliar Supply Increased Expression of NIPs Aquaporins and BOR Transporters of In Vitro Ipomoea batatas Plants. Appl. Sci. 2022, 12, 1788. [https://doi.org/10.3390/app12041788] http://hdl.handle.net/10481/73918 10.3390/app12041788 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España MDPI